Table 2 Primers useda
Primer | Sequence |
|---|---|
DF1 | 5' AAGCT(A/C/G/T) GG(A/C/G/T) G(C/T)(A/C/G/T) CCT T(C/T)(C/T) TATTGTTT(C/T) CACGAC 3' |
DR2 | 5' TCCTCC(A/C/G/T) TGCTG(C/T)(T/A)(T/A) GCCGCC(A/C/G/T) CC(A/C/G/T) CG(A/C/G/T) AG(A/G) AT 3' |
DF2 | 5' GGT(A/G) T(A/C/G/T) AA(A/G) CT(A/C/G/T) CTCTGGGG(A/C/G/T) CAGGCCAA(C/T) CT(A/C/G/T) TT(C/T) 3' |
DR1 | 5' (A/G) TG(C/T) TTCTGGGG(C/T) TC(C/T) TGCGGCTTGGGCTC (A/C/G/T) A(T/G) 3' |
pRSETBF | 5' GGTGGACAGCAAATGGGTCGG 3' |
pRSETBR | 5' GGGCTTTGTTAGCAGCCGGATC 3' |
XYM1F | 5' CCTGGATCC ATGAGCGTTGTTCTTGGCGACAAAG 3' |
XYM1R | 5' TGGGTCGAC TTACCGGATCCACCGGTTCATAATG 3' |
XYM2F | 5'CAGGAATTC ATGAAACTTACCGTAGGAGACAAGG 3' |
XYM2R | 5' TGGGTCGAC TTAAATAAACCTGCTAATCAAATTCTCAATATAC 3' |