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Table 3 Reported specific activity and maximum specific growth rate for XI genes cloned in Saccharomyces cerevisiaea

From: Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library

Source of XI Activity (U/mg protein) Aerobic growth rate on xylose (hour-1) Source Identity with xym1 XI Identity with xym2 XI Identity with Piromyces XI
Metagenome xym1b 0.33 ± 0.05 0.02 This study 100% 67% 61%
Metagenome xym2b 0.20 ± 0.04 0.02 This study 67% 100% 63%
Piromyces sp. b,c 0.35 0.07 This study 61% 63% 100%
Piromyces sp. 0.3 to 1.1 0.22 [18] 61% 63% 100%
Piromyces sp.c 0.5 to 0.8 0.02 [17] 61% 63% 100%
Piromyces sp. c 0.0538 0.056 [16] 61% 63% 100%
Orpinomyces sp. 1.6 0.01 [15] 61% 61% 94%
Xanthomonas campestris 0.016 0.02 [44] 64% 63% 61%
Bacteroides thetaiotaomicron 0.14 n.m. [45] 61% 64% 83%
Clostridium phytofermentans c 0.0344 0.057 [16] 54% 52% 54%
Clostridium thermosulfurogenes n.m. n.d. [46] 51% 49% 49%
Escherichia coli n.m. n.d. [19] 50% 52% 48%
Bacillus subtilis n.m. n.m. [47] 48% 48% 47%
Lactobacillus pentosus n.d. n.d. [47] 48% 47% 46%
Thermus thermophilus 0.0007 to 0.012 n.m. [48] 31% 30% 29%
  1. an.m., not measured; n.d., not detected. Sequence identity with xym1, xym2 and Piromyces XI gene is also given at the protein level. bGlucose-grown cells. cCodon-optimised gene.