A versatile toolkit for high throughput functional genomics with Trichoderma reesei

Table 1 Overview about selected T. reesei genes.

protein ID	gene name	function	classification/group	ORF length [bp]	protein size [aa]	successful transformation method
2526	pkc1	protein kinase C	signal transduction	3888	1140	P
2845	gna2	g-protein alpha subunit two	signal transduction	1510	355	E
3873	pde1	hypothetical cyclic AMP phosphodiesterase, class II	signal transduction	2177	526	P
21505	gna3	g-protein alpha subunit three	signal transduction	1423	356	E
23171		nonribosomal peptide synthetase (NRPS)	secondary metabolism	69518	20874	N
28731	gpr2	hypothetical GPCR, family 2	G-protein coupled receptor	1258	374	E
37515	rid1	DNA methylase, required for RIP (GenBank Accession number: JN227866)	sexual development	1815	553	E
56684	-	sugar transporter	transport	1758	504	P
58456	-	Zn2Cys6 transcription factor	transcription factor	1589	445	P
65315	-	basic leucine zipper transcription factor	transcription factor	1046	299	P
70351	-	Zn2Cys6 transcription factor	transcription factor	1670	480	P
72004	-	hypothetical GPCR, family 2,	G-protein coupled receptor	1464	442	N
77795	lim1	putative E3 ubiquitin ligase	signal transduction	2004	637	E
79202	-	hypothetical transmembrane protein, transport protein	transport	1575	401	E
79756	fwd1	hypothetical F-Box/WD40 repeat protein	circadian rhythm	2832	920	P
102497	-	Zn2Cys6 transcription factor	transcription factor	2328	665	P
102655	pde2	hypothetical phosphodiesterase	signal transduction	2933	857	P
109088	hpo1	heterochromatin regulator	chromatin binding	1308	272	P
109146	-	PTH11-GPCR	G-protein coupled receptor	1604	452	P
123786	-	non-ribosomal peptide synthetase	secondary metabolism	50787	16535	P

Protein ID's were obtained from the T. reesei Database v2.0. (P: protoplast transformation, E: electroporation of T. reesei spores, N: not successful)

ISSN: 2731-3654