Verification of the inducible marker-free and RNAi system in Jatropha. (A) Schematic diagram showing structure features of the inducible pX7-FAD2-1i and pX8-FAD2-1i constructs and Cre/loxP-mediated DNA recombination. For details, see [14, 15]. (B) Genotypic analysis of T0 transgenic shoots #1-1 and #1-2. The plasmid DNA of pX7-FAD2-1i containing HPT gene was used as positive control (CK+). (C) GC analysis of fatty acid composition in T0 transgenic shoots #1-1, #1-2 and untransformed shoot (CK-).