Figure 5
Pentose utilization by the Δ hxk/glk -strain in the presence of different D-glucose concentrations with overexpression of pentose-transporting hexose transporters. The strain TSY10-XT was inoculated in shake flasks at 30°C with 0.2 g (dry biomass)/L in SC medium with D-xylose (10 g/L) without or with 0,5 g/L 2-DOG (2DOG) and increasing D-glucose concentrations (0 - 30 g/L) in the presence of 2-DOG (0.5 g/L). Growth was determined by measuring the OD600 nm (A) and sugar concentrations were analyzed by HPLC. D-xylose consumption at different D-glucose concentrations is displayed as percentage of the total initial D-xylose consumed after 100 h (B). The strain TSY10-AT was inoculated in shake flasks at 30°C with 0.2 g (dry biomass)/L in SC medium with L-arabinose (10 g/L) without or with 0,5 g/L 2-DOG (2DOG) and increasing D-glucose concentrations (0 - 30 g/L) in the presence of 2-DOG (0.5 g/L). Growth was determined by measuring OD600 nm (C) and sugar concentrations were analyzed by HPLC. L-arabinose consumption at different D-glucose concentrations is displayed as percentage of the total initial L-arabinose consumed after 80 h (D).