Skip to main content

Table 1 S.cerevisiae strains and plasmids used in this study

From: Competition between pentoses and glucose during uptake and catabolism in recombinant Saccharomyces cerevisiae

S. cerevisiae strain or plasmid Relevant genotype or phenotype Source or reference
Strains   
CEN.PK2-1 C MAT a leu2-3,112 ura3-52 trp1-289 his3- _1 MAL2-8 c SUC2 EUROSCARF, Frankfurt
BWY1 MAT a leu2-3,112 ura3-52 trp1-289 his3- _1 MAL2-8 c SUC2; unknown beneficial mutations for L-arabinose utilization [11]
BWY1-X BWY1 with plasmids YEplac181_opt.Xi, p423pPGK1-XKS Tr This work
BWY1-A BWY1 with plasmids p423H7-synthIso, p424H7-synthKin, p425H7-synthEpi This work
BWY1-XT BWY1 with plasmids YEplac181_opt.Xi, p423pPGK1-XKS Tr, p426_HXT7 This work
BWY1-AT BWY1 with plasmids p423H7-synthIso, p424H7-synthKin, p425H7-synthEpi and pHL125re This work
TSY10 MAT a leu2-3,112 ura3-52 trp1-289 his3- _1 MAL2-8 c SUC2 Δhxk1::loxP
Δglk1::loxP Δhxk2::loxP; unknown beneficial mutations for L-arabinose utilization
This work
TSY10-X TSY10 with plasmids YEp181_pHXT7-optXI_Clos and p423pPGK1-XKS Tr This work
TSY10-XT TSY10 with plasmids YEp181_pHXT7-optXI_Clos, p423pPGK1-XKS Tr and p426_HXT7 This work
TSY10-A TSY10 with plasmids p423H7-synthIso, p424H7-synthKin and p425H7-synthEpi This work
TSY10-AT TSY10 with plasmids p423H7-synthIso, p424H7-synthKin, p425H7-synthEpi and pHL125re This work
TSY11 MAT a leu2-3,112 ura3-52 trp1-289 his3- _1 MAL2-8 c SUC2 Δhxk1::loxP
Δglk1::loxP Δhxk2::loxP Δylr446w::loxp-kanMX-loxp; unknown beneficial mutations for L-arabinose utilization
This work
TSY11-XTH TSY11 with plasmids YEp181_pHXT7-optXI_Clos, p423pPGK1-XKS Tr, p426_HXT7 and pRS314pUBR2-HXK1 This work
Plasmids   
p426H7 2 μ-plasmid; URA3 marker gene [10]
YEpkHXT7 DNA-template for amplification of HXT7 [61]
p426_HXT7 2 μ-plasmid; HXT7 under control of shortened HXT7-promoter and CYC1-terminator; URA3 marker gene This work
pHL125re 2 μ-plasmid; GAL2 under control of ADH1- pomoter, URA3 marker gene [62]
p423H7-synthIso 2 μ-plasmid; codon-optimized araA of B. licheniformis under control of shortened HXT7-promoter and CYC1-terminator; HIS3 marker gene [11]
p424H7-synthKin 2 μ-plasmid; codon-optimized araB of E. coli under control of shortened HXT7-promoter and CYC1-terminator; LEU2 marker gene [11]
p425H7-synthEpi 2 μ-plasmid; codon-optimized araD of E. coli under control of shortened HXT7-promoter and CYC1-terminator; TRP1 marker gene [11]
pUG6 DNA-template for amplification of loxP-kanMX-loxP gene resistance marker [32]
pSH47 Cre-recombinase under control of GAL1 promoter; URA3 marker gene [32]
YEp181_pHXT7-optXI_Clos 2 μ-plasmid; codon-optimized xylose isomerase gene of C. phytofermentans under control of shortened HXT7-promoter and CYC1-terminator; LEU2 marker gene Boles, lab stock
p423pPGK1-XKS Tr 2 μ-plasmid; xylulokinase gene of H. jecorina under control of PGK1-promoter and CYC1-terminator; HIS3 marker gene Boles, lab stock
pRS314 Yeast centromere vector; TRP1 marker gene P. Kötter, Frankfurt, Germany
pRS314pUBR2-HXK1 Yeast centromere vector; HXK1 of S. cerevisiae behind UBR2-promoter and HXK1-terminator; TRP1 maker gene This work