Table 1 S.cerevisiae strains and plasmids used in this study

Strains

CEN.PK2-1 C

MAT a leu2-3,112 ura3-52 trp1-289 his3- _1 MAL2-8 c SUC2

EUROSCARF, Frankfurt

BWY1

MAT a leu2-3,112 ura3-52 trp1-289 his3- _1 MAL2-8 c SUC2; unknown beneficial mutations for L-arabinose utilization

[11]

BWY1-X

BWY1 with plasmids YEplac181_opt.Xi, p423pPGK1-XKS Tr

This work

BWY1-A

BWY1 with plasmids p423H7-synthIso, p424H7-synthKin, p425H7-synthEpi

This work

BWY1-XT

BWY1 with plasmids YEplac181_opt.Xi, p423pPGK1-XKS Tr, p426_HXT7

This work

BWY1-AT

BWY1 with plasmids p423H7-synthIso, p424H7-synthKin, p425H7-synthEpi and pHL125^re

This work

TSY10

MAT a leu2-3,112 ura3-52 trp1-289 his3- _1 MAL2-8 c SUC2 Δhxk1::loxP

Δglk1::loxP Δhxk2::loxP; unknown beneficial mutations for L-arabinose utilization

This work

TSY10-X

TSY10 with plasmids YEp181_pHXT7-optXI_Clos and p423pPGK1-XKS Tr

This work

TSY10-XT

TSY10 with plasmids YEp181_pHXT7-optXI_Clos, p423pPGK1-XKS Tr and p426_HXT7

This work

TSY10-A

TSY10 with plasmids p423H7-synthIso, p424H7-synthKin and p425H7-synthEpi

This work

TSY10-AT

TSY10 with plasmids p423H7-synthIso, p424H7-synthKin, p425H7-synthEpi and pHL125^re

This work

TSY11

MAT a leu2-3,112 ura3-52 trp1-289 his3- _1 MAL2-8 c SUC2 Δhxk1::loxP

Δglk1::loxP Δhxk2::loxP Δylr446w::loxp-kanMX-loxp; unknown beneficial mutations for L-arabinose utilization

This work

TSY11-XTH

TSY11 with plasmids YEp181_pHXT7-optXI_Clos, p423pPGK1-XKS Tr, p426_HXT7 and pRS314pUBR2-HXK1

This work

Plasmids

p426H7

2 μ-plasmid; URA3 marker gene

[10]

YEpkHXT7

DNA-template for amplification of HXT7

[61]

p426_HXT7

2 μ-plasmid; HXT7 under control of shortened HXT7-promoter and CYC1-terminator; URA3 marker gene

This work

pHL125^re

2 μ-plasmid; GAL2 under control of ADH1- pomoter, URA3 marker gene

[62]

p423H7-synthIso

2 μ-plasmid; codon-optimized araA of B. licheniformis under control of shortened HXT7-promoter and CYC1-terminator; HIS3 marker gene

[11]

p424H7-synthKin

2 μ-plasmid; codon-optimized araB of E. coli under control of shortened HXT7-promoter and CYC1-terminator; LEU2 marker gene

[11]

p425H7-synthEpi

2 μ-plasmid; codon-optimized araD of E. coli under control of shortened HXT7-promoter and CYC1-terminator; TRP1 marker gene

[11]

pUG6

DNA-template for amplification of loxP-kanMX-loxP gene resistance marker

[32]

pSH47

Cre-recombinase under control of GAL1 promoter; URA3 marker gene

[32]

YEp181_pHXT7-optXI_Clos

2 μ-plasmid; codon-optimized xylose isomerase gene of C. phytofermentans under control of shortened HXT7-promoter and CYC1-terminator; LEU2 marker gene

Boles, lab stock

p423pPGK1-XKS Tr

2 μ-plasmid; xylulokinase gene of H. jecorina under control of PGK1-promoter and CYC1-terminator; HIS3 marker gene

Boles, lab stock

pRS314

Yeast centromere vector; TRP1 marker gene

P. Kötter, Frankfurt, Germany

pRS314pUBR2-HXK1

Yeast centromere vector; HXK1 of S. cerevisiae behind UBR2-promoter and HXK1-terminator; TRP1 maker gene

This work