Table 2 Primers used for gene deletions

Δ hxk1for

5'-ATGGTTCATTTAGGTCCAAAGAAACCACAGGCTAGAAAGGGTTCCATGGCTTCGTACGCTGCAGGTCGAC-3'

Δhxk1rev

5'-TTAAGCGCCAATGATACCAAGAGACTTACCTTCGGCAATTCTTTTTTCGG GCATAGGCCACTAGTGGATCTG-3'

Δglk1for

5'-ATGTCATTCGACGACTTACACAAAGCCACTGAGAGAGCGGTCATCCAGGC TTCGTACGCTGCAGGTCGAC-3'

Δglk1rev

5'-TCATGCTACAAGCGCACACAAGGCGGCACCCACTCCGGAACCATCCTTGG GCATAGGCCACTAGTGGATCTG-3'

Δhxk2for

5'-ATGGTTCATTTAGGTCCAAAAAAACCACAAGCCAGAAAGGGTTCCATGGC TTCGTACGCTGCAGGTCGAC-3'

Δhxk2rev

5'-TTAAGCACCGATGATACCAACGGACTTACCTTCAGCAATTCTTTTTTGGG GCATAGGCCACTAGTGGATCTG-3'

Δylr446wfor

5'-ATGACAATTGAAAGCACTCTAGCTCGGGAATTAGAAAGCTTGATTTTACC TTCGTACGCTGCAGGTCGAC-3'

Δylr446wrev

5'-TTATTGAACTTGGTTGTCTGATTTGTTCAAGTAGGTGGCTATTGCAGCGCC GCATAGGCCACTAGTGGATCTG-3'