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Table 4 Designed medium formulation for the verification experimenta by submerged fermentation of Streptomyces cinnamomensis

From: Controlling the simultaneous production of laccase and lignin peroxidase from Streptomyces cinnamomensisby medium formulation

 

Expected maximum activity

Yeast extract

Coffee pulp

Wheat bran

Sawdust

Urea

Total

Laccase

0.0174

0.62b (1.24%)g

1.15b (2.3%)

0.73b (1.46%)

0.89b (1.78%)

1.61b (3.22%)

5 (10%)

LiP

0.1339

0.74c (1.48%)

0.97c (1.94%)

0.19c (0.38%)

0.61c (1.22%)

2.49c (4.98%)

5 (10%)

Designed laccase:LiP ratio

      

   0.1 laccase: 1 LiP

-

0.74d

0.98d

0.20d

0.61d

2.47d

5

   1 laccase: 1 LiP

-

0.76e

1.01e

0.28e

0.66e

2.29e

5

   2 laccase: 1 LiP

-

0.77f

1.03f

0.35f

0.69f

2.16f

5

  1. aSee Methods section for details.
  2. bSolutions to max (laccase) in Table 2.
  3. cSolutions to max (LiP) in Table 2.
  4. dSolutions to max (0.1 × laccase + 1 × lLiP) in Table 3.
  5. eSolutions to max (1 × llaccase + 1 × lLiP) in Table 3.
  6. fSolutions to max (2 × llaccase + 1 × lLiP) in Table 3.
  7. g1.24% = (0.62 g yeast extract)/(50 mL liquid medium) × 100%, the remaining percentage data in this table was calculated similarly.