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Table 4 Designed medium formulation for the verification experimenta by submerged fermentation of Streptomyces cinnamomensis

From: Controlling the simultaneous production of laccase and lignin peroxidase from Streptomyces cinnamomensisby medium formulation

  Expected maximum activity Yeast extract Coffee pulp Wheat bran Sawdust Urea Total
Laccase 0.0174 0.62b (1.24%)g 1.15b (2.3%) 0.73b (1.46%) 0.89b (1.78%) 1.61b (3.22%) 5 (10%)
LiP 0.1339 0.74c (1.48%) 0.97c (1.94%) 0.19c (0.38%) 0.61c (1.22%) 2.49c (4.98%) 5 (10%)
Designed laccase:LiP ratio       
   0.1 laccase: 1 LiP - 0.74d 0.98d 0.20d 0.61d 2.47d 5
   1 laccase: 1 LiP - 0.76e 1.01e 0.28e 0.66e 2.29e 5
   2 laccase: 1 LiP - 0.77f 1.03f 0.35f 0.69f 2.16f 5
  1. aSee Methods section for details.
  2. bSolutions to max (laccase) in Table 2.
  3. cSolutions to max (LiP) in Table 2.
  4. dSolutions to max (0.1 × laccase + 1 × lLiP) in Table 3.
  5. eSolutions to max (1 × llaccase + 1 × lLiP) in Table 3.
  6. fSolutions to max (2 × llaccase + 1 × lLiP) in Table 3.
  7. g1.24% = (0.62 g yeast extract)/(50 mL liquid medium) × 100%, the remaining percentage data in this table was calculated similarly.