Figure 4

PCR analysis of the genotype of Synechocystis sp. PCC 6803 mutant strains. (A) lane1: DNA marker (1 kb DNA Ladder Marker), lane2: genomic DNA of wild-type was amplified by primers 0168-1 and far-1 (control), lane3: genomic DNA of GQ5 was amplified by the same primers as lane2, lane4: plasmid pXT14 was amplified by the same primers as lane2, lane5: genomic of wild-type was amplified by primers 0168-1 and 0168-2, lane6: genomic DNA of GQ5 was amplified by the same primers as lane5, lane7: plasmid pXT14 was amplified by the same primer as lane5 (control), lane8: genomic DNA of wild-type was amplified by primers pD1-3 and 1609R (control), lane9: genomic DNA of GQ5 was amplified by the same primers as lane8, lane10: plasmid pGQ49 was amplified by the same primers as lane8 (control), lane11: genomic DNA of wild-type was amplified by primers pD1-3 and pD1-2d-2 (control), lane12: genomic DNA of GQ5 was amplified by the same primers as lane11, lane13: plasmid pGQ49 was amplified by the same primers as lane11 (control). (B) lane1: DNA marker (1 kb DNA Ladder Marker), lane2: genomic DNA of wild-type was amplified by the primers 0168-1 and far-1 (control), lane3: genomic DNA of GQ6 was amplified by the same primers as lane2, lane4: plasmid pXT14 was amplified by the same primers as lane2 (control), lane5: genomic DNA of wild-type was amplified by primers 0168-1 and 0168-2 (control), lane6: genomic DNA of GQ6 was amplified by the same primers as lane5, lane7: plasmid pXT14 was amplified by the same primer as lane5 (control), lane8: genomic DNA of wild-type was amplified by primer 1609NdeI and 1609R (control, the size of target DNA fragment should be 2091 bp), lane9: genomic DNA of GQ6 was amplified by the same primers as lane8 (the size of target DNA fragment should be 1810 bp), lane10: plasmid pGQ17 was amplified by the same primers as lane8 (control, the size of target DNA fragment should be 1810 bp).