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Table 1 Plasmids constructed and used in this study

From: Effects of fatty acid activation on photosynthetic production of fatty acid-based biofuels in Synechocystis sp. PCC6803

Plasmid Relevant characteristicsa, b Reference
pGQ7 Apr, pET21b derivative containing slr1609gene, T7 promoter This study
pXT25 Apr, pMD18-T derivative containing upstream fragment of psbA2, T7 promoter This study
pXT59 Apr, pMD18-T derivative containing downstream fragment of psbA2, T7 promoter This study
pXT62 Apr, Kanr, pMD18-T derivative containing upstream fragment of psbA2 and CK2 This study
pXT68 Apr, Kanr, pMD18-T derivative containing upstream and downstream fragments of psbA2, CK2 and sacB This study
pFQ20 Apr Sper, pKW1188SL derivative containing lacZ, Prbc promoter [4]
pGQ11 Apr Sper , pFQ20 derivative containing slr1609, Prbc promoter This study
pGQ49 Kanr, pXT68 derivative containing slr1609, PpsbA2 promoter This study
pGQ12 Apr, pMD18-T derivative containing upstream fragment of slr1609, T7 promoter This study
pGQ13 Apr, pMD18-T derivative containing downstream fragment of slr1609, T7 promoter This study
pGQ14 Apr, Kanr, pMD18-T derivative containing upstream fragment of slr1609 and CK2 This study
pGQ17 Apr, Kanr, pMD18-T derivative containing upstream and downstream fragments of slr1609 and CK2 This study
pGQ53 Apr, Cmr, Emr, pMD18-T derivative containing upstream and downstream fragments of slr1609, CCEII and sacB This study
pXT14 Sper, pFQ20 derivative containing FAR gene (jojoba), Prbc promoter [4]
  1. a Ap, Ampicillin. Spe, Spectinomycin. Kan, kanamycin. Cm, Chloramphenicol. Em, Erythromycin.
  2. bCK2 gene for kanamycin antibiotic resistance and CCEII gene for erythromycin antibiotic resistance.