Table 1 Plasmids constructed and used in this study

pGQ7

Ap^r, pET21b derivative containing slr1609gene, T7 promoter

This study

pXT25

Ap^r, pMD18-T derivative containing upstream fragment of psbA2, T7 promoter

This study

pXT59

Ap^r, pMD18-T derivative containing downstream fragment of psbA2, T7 promoter

This study

pXT62

Ap^r, Kan^r, pMD18-T derivative containing upstream fragment of psbA2 and CK2

This study

pXT68

Ap^r, Kan^r, pMD18-T derivative containing upstream and downstream fragments of psbA2, CK2 and sacB

This study

pFQ20

Ap^r Spe^r, pKW1188SL derivative containing lacZ, P_rbc promoter

[4]

pGQ11

Ap^r Spe^r , pFQ20 derivative containing slr1609, P_rbc promoter

This study

pGQ49

Kan^r, pXT68 derivative containing slr1609, P_psbA2 promoter

This study

pGQ12

Ap^r, pMD18-T derivative containing upstream fragment of slr1609, T7 promoter

This study

pGQ13

Ap^r, pMD18-T derivative containing downstream fragment of slr1609, T7 promoter

This study

pGQ14

Ap^r, Kan^r, pMD18-T derivative containing upstream fragment of slr1609 and CK2

This study

pGQ17

Ap^r, Kan^r, pMD18-T derivative containing upstream and downstream fragments of slr1609 and CK2

This study

pGQ53

Ap^r, Cm^r, Em^r, pMD18-T derivative containing upstream and downstream fragments of slr1609, CCEII and sacB

This study

pXT14

Spe^r, pFQ20 derivative containing FAR gene (jojoba), P_rbc promoter

[4]