|
Compound
|
Cell pellets: abundance ratio to wild-type
|
Culture supernatants: abundance ratio to wild-type
|
|---|
| |
mdh
|
ldh
|
mdh
-
ldh
|
mdh
|
ldh
|
mdh
-
ldh
|
|---|
|
Malic acid
|
0.32
|
1.48
|
0.28
|
0.84
|
1.44
|
0.57
|
|
2-Hydroxyglutaric acid
|
0.28
|
1.42
|
12.43
|
0.35
|
1.72
|
4.94
|
|
Valine
|
0.27
|
1.01
|
0.37
|
0.35
|
1.17
|
0.68
|
|
Glucose-1-P
|
0.90
|
1.20
|
0.56
|
0.89
|
1.36
|
0.57
|
|
Fructose-6-P
|
0.70
|
0.99
|
0.79
|
0.99
|
2.89
|
1.39
|
|
Glucose-6-P
|
0.39
|
1.11
|
1.12
|
1.41
|
9.21
|
4.69
|
|
Succinic acid
|
0.51
|
0.56
|
1.12
|
0.58
|
0.92
|
0.62
|
|
Fumaric acid
|
0.31
|
0.70
|
0.40
|
0.48
|
0.79
|
0.36
|
|
p-Hydroxybenzoic acid
|
0.53
|
0.65
|
1.24
|
0.46
|
0.98
|
1.77
|
|
10.13 191 246 376 348
|
0.22
|
0.88
|
0.25
|
0.35
|
1.13
|
0.90
|
|
Citric acid
|
0.12
|
0.96
|
6.03
|
ND
|
ND
|
ND
|
|
11.05 191 276
|
0.44
|
1.53
|
0.18
|
ND
|
ND
|
ND
|
|
4-Methyl-2-hydroxypentanoic acid
|
ND
|
ND
|
ND
|
0.17
|
0.91
|
2.63
|
|
Pyruvic acid
|
0.56
|
0.45
|
0.88
|
0.18
|
2.25
|
0.95
|
|
Galactose
|
ND
|
ND
|
ND
|
3.41
|
2.67
|
2.32
|
|
2,3-Butanediol
|
ND
|
ND
|
ND
|
0.29
|
1.76
|
1.92
|
|
9.97 331 359 226
|
ND
|
ND
|
ND
|
0.11
|
1.62
|
2.12
|
|
11.07 191 348 320 246
|
ND
|
ND
|
ND
|
0.73
|
1.46
|
0.44
|
- Metabolites were analyzed by gas chromatography-mass spectrometry (GC-MS) as trimethylsilyl (TMS) derivatives, using a sorbitol internal standard [42]. Peaks that could not be assigned to a standard metabolite are identified by their chromatographic retention times (in min) and their characteristic ion peaks (m/z in italic text).
- ND = not determined (the concentrations of these metabolites were too low to produce reliable estimates of relative abundance).
