Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity

Table 2 Domain-level primers used to amplify short-subunit rDNA genes from DNA extracts of biomass compost

	Genes	Primer sequences & reference	Amplicon size(bp)
Archaea	16 s rDNA	F: TTCCGGTTGATCCYGCCRG R: YCCGGCGTTGAMTCCAATT Ref: [22]	937
Bacteria	16 s rDNA	F: TCCTACGGGAGGCAGCAGT R: GGACTACCAGGGTATCTAATCCTGTT Ref: [25]	466
Fungi	5.8 s and ITS2 rDNA	F: GCATCGATGAAGAACGCAGC R: TCCTCCGCTTATTGATATGC Ref: [22, 26]	400

ISSN: 2731-3654