Figure 2From: Two structurally discrete GH7-cellobiohydrolases compete for the same cellulosic substrate fiberOnly Cbh1 binds to cellulose. 20 μg of Cbh1, CelD or both (Cbh1/CelD) proteins (S) were incubated with 30% Avicel for 30 min, the slurry centrifuged, supernatants (F, free enzyme fraction) collected and enzymes removed from the pellet (B, cellulose-bound proteins) with 1% SDS and β-mercaptoethanol, concentrated and analyzed by SDS-PAGE.Back to article page