Two structurally discrete GH7-cellobiohydrolases compete for the same cellulosic substrate fiber

Table 2 Cbh1 and CelD kinetic properties on crystalline cellulosic substrates

Kinetic parameter	Avicel		Cotton cellulose		pNPC
	Cbh1	CelD	Cbh1	CelD	Cbh1	CelD
V_max(μmol/min)	24.81±0.75	3.39±0.35	20.62±0.62	3.95±0.06	-	-
K_m(mg/mL)	18.27±1.97	43.50±6.39	18.83±1.99	16.60±0.97	-	-
K_cat(min^-1)	22.20	2.62	18.45	3.05	-	-
K_cat/K_m	1.22	0.06	0.98	0.18	-	-
U mg prot^-1*	7.66±1.21	0.49±0.04	4.57±0.02	1.19±0.06	26.20±0.20	27.31±1.40

^*-Specific activity (U.mg prot^-1) determined with 10 mg/ml avicel cotton cellulose or 18 mM p NPC at 40 C FOR 120 mins.

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