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Table 2 Cbh1 and CelD kinetic properties on crystalline cellulosic substrates

From: Two structurally discrete GH7-cellobiohydrolases compete for the same cellulosic substrate fiber

Kinetic parameter Avicel Cotton cellulose pNPC
  Cbh1 CelD Cbh1 CelD Cbh1 CelD
Vmax(μmol/min) 24.81±0.75 3.39±0.35 20.62±0.62 3.95±0.06 - -
Km(mg/mL) 18.27±1.97 43.50±6.39 18.83±1.99 16.60±0.97 - -
Kcat(min-1) 22.20 2.62 18.45 3.05 - -
Kcat/Km 1.22 0.06 0.98 0.18 - -
U mg prot-1* 7.66±1.21 0.49±0.04 4.57±0.02 1.19±0.06 26.20±0.20 27.31±1.40
  1. *-Specific activity (U.mg prot-1) determined with 10 mg/ml avicel cotton cellulose or 18 mM p NPC at 40 C FOR 120 mins.