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Table 1 Summary of stepwise purification of NpaBGS (β-glucosidase)

From: A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5

Purification steps Total activity (U) Total protein (mg) Specific activity (U/mg) Purification (fold) Recover rate (%)
Supernatant 2396 198 12.1 1 100
Condensation 1900 96 19.8 1.6 79
Ammonium sulfate precipitation 985 47 21.0 1.7 41
1 st DEAE-Sepharose chromatography 524 30 17.5 1.5 22
2 nd DEAE-Sepharose chromatography 345 10 34.5 2.9 14
  1. The assay was conducted at 40°C, pH 6 for 1 hr. One unit enzyme activity was defined as 1 nmole of glucose released per minute.