From: A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5
Purification steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Recover rate (%) |
---|---|---|---|---|---|
Supernatant | 2396 | 198 | 12.1 | 1 | 100 |
Condensation | 1900 | 96 | 19.8 | 1.6 | 79 |
Ammonium sulfate precipitation | 985 | 47 | 21.0 | 1.7 | 41 |
1 st DEAE-Sepharose chromatography | 524 | 30 | 17.5 | 1.5 | 22 |
2 nd DEAE-Sepharose chromatography | 345 | 10 | 34.5 | 2.9 | 14 |