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Table 1 Summary of stepwise purification of NpaBGS (β-glucosidase)

From: A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5

Purification steps

Total activity (U)

Total protein (mg)

Specific activity (U/mg)

Purification (fold)

Recover rate (%)

Supernatant

2396

198

12.1

1

100

Condensation

1900

96

19.8

1.6

79

Ammonium sulfate precipitation

985

47

21.0

1.7

41

1 st DEAE-Sepharose chromatography

524

30

17.5

1.5

22

2 nd DEAE-Sepharose chromatography

345

10

34.5

2.9

14

  1. The assay was conducted at 40°C, pH 6 for 1 hr. One unit enzyme activity was defined as 1 nmole of glucose released per minute.