A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5

Table 2 Substrate specificity of purified NpaBGS

Substrate	Relative activity (%)
Amygdalin	92
Sinigrin	1
Esculin	87
Phenyl-β-D-glucoside	99
Arbutin	95
β-methyl-D-glucoside	11
Cellobiose	100
β-Gentiobiose	93
Maltose	0
Lactose	0
Sucrose	0
Laminarin	98
4-methylumbelliferyl-β-D-cellobioside	265
4-methylumbelliferyl-β-D-glucopyranoside	255
4-mehtylumbelliferyl-β-D- galactopyronoside	0

The assay was performed at 40°C, pH 6 for 1 hr using 0.01units/ml NpaBGS enzyme. The relative activities are expressed as percentage by normalizing to the cellobiose reaction activity.

ISSN: 2731-3654