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Table 2 Substrate specificity of purified NpaBGS

From: A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5

Substrate Relative activity (%)
Amygdalin 92
Sinigrin 1
Esculin 87
Phenyl-β-D-glucoside 99
Arbutin 95
β-methyl-D-glucoside 11
Cellobiose 100
β-Gentiobiose 93
Maltose 0
Lactose 0
Sucrose 0
Laminarin 98
4-methylumbelliferyl-β-D-cellobioside 265
4-methylumbelliferyl-β-D-glucopyranoside 255
4-mehtylumbelliferyl-β-D- galactopyronoside 0
  1. The assay was performed at 40°C, pH 6 for 1 hr using 0.01units/ml NpaBGS enzyme. The relative activities are expressed as percentage by normalizing to the cellobiose reaction activity.