Figure 2From: Production and effect of aldonic acids during enzymatic hydrolysis of lignocellulose at high dry matter contentSeparation of sugars and oxidized sugars with HPAEC and HPLC systems. Chromatograms from HPAEC with PAD ( A) and HPLC equipped with both RI ( B) and UV ( C) detector connected in series. In all chromatograms are shown separation of a standard sample containing cellobionic acid, cellobiose, gluconic acid and glucose (dotted line) and a sample from hydrolysis of pretreated wheat straw (solid line). The pretreated wheat straw sample was hydrolyzed for 48 hours at 50°C with Cellic CTec2 at enzyme loading of 10 mg/g DM of biomass at 30% of WIS. GlcGlcA: cellobionic acid, GlcA: gluconic acid, GlcGlc: cellobiose, Glc: glucose.Back to article page