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Figure 2 | Biotechnology for Biofuels

Figure 2

From: Production and effect of aldonic acids during enzymatic hydrolysis of lignocellulose at high dry matter content

Figure 2

Separation of sugars and oxidized sugars with HPAEC and HPLC systems. Chromatograms from HPAEC with PAD ( A) and HPLC equipped with both RI ( B) and UV ( C) detector connected in series. In all chromatograms are shown separation of a standard sample containing cellobionic acid, cellobiose, gluconic acid and glucose (dotted line) and a sample from hydrolysis of pretreated wheat straw (solid line). The pretreated wheat straw sample was hydrolyzed for 48 hours at 50°C with Cellic CTec2 at enzyme loading of 10 mg/g DM of biomass at 30% of WIS. GlcGlcA: cellobionic acid, GlcA: gluconic acid, GlcGlc: cellobiose, Glc: glucose.

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