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Table 1 Experimental design and results of the CCRD matrix with observed and expected values of the bioemulsifier production and emulsifying activity

From: Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2

Run Variables/levels Biemulsifier production (mg/l) (Y1) Emulsifying activity (E24) (Y2)
  Yeast extract (g/L) ( X1) Residue biodiesel (g/L) ( X2) Ammonium sulfate (g/L) ( X3) Observerd Expected Observed Expected
1 −1.00 −1.00 −1.00 665.00 596.38 42.67 24.75
2 1.00 −1.00 −1.00 727.33 596.38 45.00 24.75
3 −1.00 1.00 −1.00 783.33 923.29 45.00 45.17
4 1.00 1.00 −1.00 841.33 923.29 45.00 45.17
5 −1.00 −1.00 1.00 3488.00 2908.37 78.33 63.07
6 1.00 −1.00 1.00 4458.67 2908.37 61.50 63.07
7 −1.00 1.00 1.00 6981.67 6174.95 61.94 83.49
8 1.00 1.00 1.00 7076.67 6174.95 80.67 83.49
9 −1.68 0.00 0.00 3517.33 2650.75 81.33 66.62
10 1.68 0.00 0.00 3859.00 2650.75 80.00 66.62
11 0.00 −1.68 0.00 0.00 1139,87 0.00 33.52
12 0.00 1.68 0.00 3523.00 4161.63 79.33 67.86
13 0.00 0.00 −1.68 0.00 −529.37 0,00 14.97
14 0.00 0.00 1.68 4064.67 5830.87 72.33 79.41
15 0.00 0.00 0.00 2194.67 2650.75 57.67 66.62
16 0.00 0.00 0.00 2205.67 2650.75 60.00 66.62
17 0.00 0.00 0.00 2189.33 2650.75 57.67 66.62
18 0.00 0.00 0.00 2145.67 2650.75 58.67 66.62
19 0.00 0.00 0.00 2159.00 2650.75 60.00 66.62
20 0.00 0.00 0.00 2134.67 2650.75 56.60 66.62
  1. Values represent means of three experimental repetitions.