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Figure 5 | Biotechnology for Biofuels

Figure 5

From: Engineering better biomass-degrading ability into a GH11 xylanase using a directed evolution strategy

Figure 5

Percentage conversion of total sugars in xylanase-depleted wheat straw (Dpl-WS) and intact wheat straw (In-WS) by Thermobacillus xylanilyticus xylanase (Tx-Xyn) alone or in combination with Accellerase 1500 . (A) Conversion of total xylose in reactions involving wild-type or mutant Tx-Xyn and Dpl-WS (reactions performed at pH 5.8) or In-WS (pH 5.0 and 5.8) as substrates. (B) Conversion of total xylose and glucose in In-WS, using Accellerase 1500 alone or in combination with wild-type and mutant Tx-Xyn (reactions performed at pH 5.0). In both (A) and (B), the x-axis shows the enzyme(s) employed in the corresponding hydrolysis reaction. The method used to derive percentage conversion is described in the Methods section.

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