Percentage conversion of total sugars in xylanase-depleted wheat straw (Dpl-WS) and intact wheat straw (In-WS) by Thermobacillus xylanilyticus xylanase (Tx-Xyn) alone or in combination with Accellerase 1500 . (A) Conversion of total xylose in reactions involving wild-type or mutant Tx-Xyn and Dpl-WS (reactions performed at pH 5.8) or In-WS (pH 5.0 and 5.8) as substrates. (B) Conversion of total xylose and glucose in In-WS, using Accellerase 1500 alone or in combination with wild-type and mutant Tx-Xyn (reactions performed at pH 5.0). In both (A) and (B), the x-axis shows the enzyme(s) employed in the corresponding hydrolysis reaction. The method used to derive percentage conversion is described in the Methods section.