|
Library type
|
Substrate
|
Variants screened
|
CV WT
|
Percentage of clones with improved activity
|
No. of hits selected
|
|---|
| | | | |
>4C
|
>5C
|
>6C
|
>7C
|
>8C
| |
|---|
| | | | |
V
|
V
|
V
|
V
|
V
| |
|---|
|
epPCR
|
In-WS
|
264
|
11.1 ± 1.3%
|
0.4%
|
0.4%
|
-
|
-
|
-
|
1
|
|
epPCR
|
In-WS
|
4,333
|
18.1 ± 5.4%
|
0.1%
|
-
|
-
|
-
|
-
|
4
|
|
SDM
|
-
|
-
|
-
| | | | | |
11
|
|
epPCR
|
Dpl-WS
|
4,300
|
10.9 ± 2.2%
|
1.2%
|
0.6%
|
-
|
-
|
-
|
30
|
|
Shuffling
|
Dpl-WS
|
3,840a (approximat ely 2,500)
|
8.1 ± 0.6%
|
1.4%
|
6.0%
|
2.1%
|
0.8%
|
0.1%
|
7
|
|
Shuffling
|
Dpl-WS
|
864a (1,847)
|
10.2%
|
9.3%
|
2.8%
|
0.9%
|
0.2
|
-
|
8
|
|
Shuffling
|
Dpl-WS
|
864a (127)
|
11.3%
|
19.5%
|
7.5%
|
2.4%
|
0.5%
|
0.2%
|
7
|
- CV = coefficient of variation; Dpl-WS = xylanase-depleted wheat straw; epPCR = error-prone PCR; In-WS = intact wheat straw; SDM = site-directed mutagenesis; WT = wild-type.
- aValues in brackets are the number of theoretical mutational combinations.
