Table 1 Summary of directed evolution for improvement of Thermobacillus xylanilyticus xylanase (Tx-Xyn) xylanase activity

epPCR

In-WS

264

11.1 ± 1.3%

0.4%

0.4%

-

-

-

1

epPCR

In-WS

4,333

18.1 ± 5.4%

0.1%

-

-

-

-

4

SDM

-

-

-

11

epPCR

Dpl-WS

4,300

10.9 ± 2.2%

1.2%

0.6%

-

-

-

30

Shuffling

Dpl-WS

3,840^a (approximat ely 2,500)

8.1 ± 0.6%

1.4%

6.0%

2.1%

0.8%

0.1%

7

Shuffling

Dpl-WS

864^a (1,847)

10.2%

9.3%

2.8%

0.9%

0.2

-

8

Shuffling

Dpl-WS

864^a (127)

11.3%

19.5%

7.5%

2.4%

0.5%

0.2%

7