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Table 1 Summary of directed evolution for improvement of Thermobacillus xylanilyticus xylanase (Tx-Xyn) xylanase activity

From: Engineering better biomass-degrading ability into a GH11 xylanase using a directed evolution strategy

Library type Substrate Variants screened CV WT Percentage of clones with improved activity No. of hits selected
     >4C >5C >6C >7C >8C  
     V V V V V  
epPCR In-WS 264 11.1 ± 1.3% 0.4% 0.4% - - - 1
epPCR In-WS 4,333 18.1 ± 5.4% 0.1% - - - - 4
SDM - - -       11
epPCR Dpl-WS 4,300 10.9 ± 2.2% 1.2% 0.6% - - - 30
Shuffling Dpl-WS 3,840a (approximat ely 2,500) 8.1 ± 0.6% 1.4% 6.0% 2.1% 0.8% 0.1% 7
Shuffling Dpl-WS 864a (1,847) 10.2% 9.3% 2.8% 0.9% 0.2 - 8
Shuffling Dpl-WS 864a (127) 11.3% 19.5% 7.5% 2.4% 0.5% 0.2% 7
  1. CV = coefficient of variation; Dpl-WS = xylanase-depleted wheat straw; epPCR = error-prone PCR; In-WS = intact wheat straw; SDM = site-directed mutagenesis; WT = wild-type.
  2. aValues in brackets are the number of theoretical mutational combinations.