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Table 2 Characteristics of intact wheat straw (In-WS) and xylanase-depleted wheat straw (Dpl-WS) and summary of the two screening assays

From: Engineering better biomass-degrading ability into a GH11 xylanase using a directed evolution strategy

  In-WS screening Dpl-WS screening
Substrate properties:   
Substrate type Intact wheat straw Xylanase-depleted wheat straw
Particle size Average 0.5 mm  
Glucose, % (w/w) 44.51 ± 0.08% 45.69 ± 0.94%
Xylose, % (w/w) 26.16 ± 0.14% 21.92 ± 0.17%
Arabinose, % (w/w) 2.37 ± 0.03% 2.05 ± 0.07%
Ratio of Ara:Xyl 0.091 0.094
Screening conditions:   
Weight (mg per microplate) 420 to 440 385 to 405
Cell-free extract (CFE) loading CFE in NaOAc, pH 5.8, 250 μl/well
Temperature and time 60°C, 4 h 60°C, 16 h
Sealing Aluminum film Polypropylene film
Evaporation (w/w, %) 1.44 ± 0.16% 0.23 ± 0.05%
Activity assay Micro-DNS assay