Table 2 Characteristics of intact wheat straw (In-WS) and xylanase-depleted wheat straw (Dpl-WS) and summary of the two screening assays
In-WS screening | Dpl-WS screening | |
|---|---|---|
Substrate properties: | ||
Substrate type | Intact wheat straw | Xylanase-depleted wheat straw |
Particle size | Average 0.5 mm | |
Glucose, % (w/w) | 44.51 ± 0.08% | 45.69 ± 0.94% |
Xylose, % (w/w) | 26.16 ± 0.14% | 21.92 ± 0.17% |
Arabinose, % (w/w) | 2.37 ± 0.03% | 2.05 ± 0.07% |
Ratio of Ara:Xyl | 0.091 | 0.094 |
Screening conditions: | ||
Weight (mg per microplate) | 420 to 440 | 385 to 405 |
Cell-free extract (CFE) loading | CFE in NaOAc, | pH 5.8, 250 μl/well |
Temperature and time | 60°C, 4 h | 60°C, 16 h |
Sealing | Aluminum film | Polypropylene film |
Evaporation (w/w, %) | 1.44 ± 0.16% | 0.23 ± 0.05% |
Activity assay | Micro-DNS assay |