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Table 3 Mutational frequency in the fifth to seventh generations

From: Engineering better biomass-degrading ability into a GH11 xylanase using a directed evolution strategy

Generation

Y6H

Y24F

S27T

S29N

N30D

L64P

W109R

Y111H

K132R

V139A

Y172H

Fifth

22.2%

11.1%

22.2%

11.1%

11.1%

22.2%

11.1%

100%

22.2%

22.2%

11.1%

Sixth

28.6%

14.3%

28.6%

-

14.3%

-

-

100%

14.3%

14.3%

-

Seventh

44.4%

-

55.6%

-

11.1%

-

-

100%

22.2%

22.2%

-