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Table 4 Thermostability of Thermobacillus xylanilyticus xylanase (Tx-Xyn) and mutants thereof

From: Engineering better biomass-degrading ability into a GH11 xylanase using a directed evolution strategy

Mutant Tm(°C) t1/2at 60°C (h)
Tx-Xyn 75.9 5.4
Y6H 72.9 2.6
S27T 76.4 6.4
Y111H 75.1 3.9
Y6H-Y111H 72.7 2.7
S27T-Y111H 75.4 6.4
Y6H-S27T-Y111H 74.3 3.9
Y3W 73.1 3.2
Y111S 75.1 3.6
Y111T 74.9 5.0
  1. The melting temperature (Tm) was determined using differential scanning fluorimetry (DSF) and the half-life (t1/2) was defined as the period necessary for the initial activity to be reduced by 50% at 60°C.