Mutant
|
Tm(°C)
|
t1/2at 60°C (h)
|
---|
Tx-Xyn
|
75.9
|
5.4
|
Y6H
|
72.9
|
2.6
|
S27T
|
76.4
|
6.4
|
Y111H
|
75.1
|
3.9
|
Y6H-Y111H
|
72.7
|
2.7
|
S27T-Y111H
|
75.4
|
6.4
|
Y6H-S27T-Y111H
|
74.3
|
3.9
|
Y3W
|
73.1
|
3.2
|
Y111S
|
75.1
|
3.6
|
Y111T
|
74.9
|
5.0
|
- The melting temperature (Tm) was determined using differential scanning fluorimetry (DSF) and the half-life (t1/2) was defined as the period necessary for the initial activity to be reduced by 50% at 60°C.