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Table 4 Thermostability of Thermobacillus xylanilyticus xylanase (Tx-Xyn) and mutants thereof

From: Engineering better biomass-degrading ability into a GH11 xylanase using a directed evolution strategy

Mutant

Tm(°C)

t1/2at 60°C (h)

Tx-Xyn

75.9

5.4

Y6H

72.9

2.6

S27T

76.4

6.4

Y111H

75.1

3.9

Y6H-Y111H

72.7

2.7

S27T-Y111H

75.4

6.4

Y6H-S27T-Y111H

74.3

3.9

Y3W

73.1

3.2

Y111S

75.1

3.6

Y111T

74.9

5.0

  1. The melting temperature (Tm) was determined using differential scanning fluorimetry (DSF) and the half-life (t1/2) was defined as the period necessary for the initial activity to be reduced by 50% at 60°C.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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