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Table 5 Kinetic parameters of Thermobacillus xylanilyticus xylanase (Tx-Xyn) and mutants for hydrolyses involving either birchwood xylan (BWX) or LVWAX

From: Engineering better biomass-degrading ability into a GH11 xylanase using a directed evolution strategy

Mutant

Kinetic parametersa

SRc

 

BWX

LVWAX

 
 

k cat (s-1)

KMb (g/l)

kcat /KMb(s-1/g/l)

k cat (s-1)

KMb (g/l)

kcat /Kmb (s-1/g/l)

 

Tx-Xyn

610.5 ± 19.6

2.54 ± 0.19

242.1

1,699.4 ± 95.9

5.10 ± 0.09

333.1

0.73

Y6H

806.1 ± 61.2

2.37 ± 0.27

340.5

2,081.6 ± 16.4

5.73 ± 0.09

363.0

0.94

S27T

742.9 ± 22.4

1.93 ± 0.15

376.5

1,936.0 ± 19.2

4.81 ± 0.04

402.5

0.94

Y111H

449.3 ± 23.2

1.54 ± 0.14

292.0

1,889.0 ± 72.9

5.01 ± 0.07

376.7

0.78

Y6H-Y111H

433.1 ± 12.5

1.91 ± 0.11

226.8

1,834.7 ± 75.4

5.33 ± 0.35

345.9

0.66

S27T-Y111H

535.8 ± 30.2

1.72 ± 0.21

311.5

1,906.1 ± 4.4

4.39 ± 0.02

434.1

0.72

Y3W

704.5 ± 29.8

2.11 ± 0.14

333.4

1,743.0 ± 26.5

5.51 ± 0.02

316.3

1.05

Y111S

758.8 ± 15.9

2.12 ± 0.04

358.6

1,755.0 ± 106.3

4.75 ± 0.14

369.4

0.97

Y111T

905.2 ± 17.2

2.35 ± 0.15

369.4

1,740.4 ± 41.8

4.81 ± 0.06

361.4

1.02

  1. aAll data presented are the result of triplicate experiments.
  2. bThe heterogeneous nature of the substrate excludes the determination of a value for KM , thus values are apparent.
  3. cRatio of the performance constants k c a t B W X / K M B W X / k c a t L V W a X / K M L V W A X for a given enzyme.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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