|
Mutant
|
Kinetic parametersa
|
SRc
|
|---|
| |
BWX
|
LVWAX
| |
|---|
| |
k
cat
(s-1)
|
KMb (g/l)
|
kcat /KMb(s-1/g/l)
|
k
cat
(s-1)
|
KMb (g/l)
|
kcat /Kmb (s-1/g/l)
| |
|---|
|
Tx-Xyn
|
610.5 ± 19.6
|
2.54 ± 0.19
|
242.1
|
1,699.4 ± 95.9
|
5.10 ± 0.09
|
333.1
|
0.73
|
|
Y6H
|
806.1 ± 61.2
|
2.37 ± 0.27
|
340.5
|
2,081.6 ± 16.4
|
5.73 ± 0.09
|
363.0
|
0.94
|
|
S27T
|
742.9 ± 22.4
|
1.93 ± 0.15
|
376.5
|
1,936.0 ± 19.2
|
4.81 ± 0.04
|
402.5
|
0.94
|
|
Y111H
|
449.3 ± 23.2
|
1.54 ± 0.14
|
292.0
|
1,889.0 ± 72.9
|
5.01 ± 0.07
|
376.7
|
0.78
|
|
Y6H-Y111H
|
433.1 ± 12.5
|
1.91 ± 0.11
|
226.8
|
1,834.7 ± 75.4
|
5.33 ± 0.35
|
345.9
|
0.66
|
|
S27T-Y111H
|
535.8 ± 30.2
|
1.72 ± 0.21
|
311.5
|
1,906.1 ± 4.4
|
4.39 ± 0.02
|
434.1
|
0.72
|
|
Y3W
|
704.5 ± 29.8
|
2.11 ± 0.14
|
333.4
|
1,743.0 ± 26.5
|
5.51 ± 0.02
|
316.3
|
1.05
|
|
Y111S
|
758.8 ± 15.9
|
2.12 ± 0.04
|
358.6
|
1,755.0 ± 106.3
|
4.75 ± 0.14
|
369.4
|
0.97
|
|
Y111T
|
905.2 ± 17.2
|
2.35 ± 0.15
|
369.4
|
1,740.4 ± 41.8
|
4.81 ± 0.06
|
361.4
|
1.02
|
- aAll data presented are the result of triplicate experiments.
- bThe heterogeneous nature of the substrate excludes the determination of a value for KM , thus values are apparent.
- cRatio of the performance constants for a given enzyme.
