Figure 1

Plasmid maps of pAMG205 and pAMG206. The only difference between these plasmids is the presence of either the C. thermocellum pyrF gene or the T. saccharolyticum hpt gene. Dcm methylation sites are highlighted. CYC1 term, Saccharomyces cerevisiae CYC1 transcriptional terminator; pUC origin, origin of replication from pUC19; bla, b-lactamase; URA3, Saccharomyces cerevisiae URA3 gene; CEN6/ARSH4, low copy origin of replication in Saccharomyces cerevisiae; pNW33N ori, origin of replication from pNW33N; repB, encodes putative protein responsible for plasmid replication initiation; putative mob/pre fragment, encodes putative protein with homology to the Mob/Pre family involved in plasmid mobilization and recombination; P-gapD, promoter region of the C. thermocellum gapD gene; cat, chloramphenicol acetyltransferase; pyrF, C. thermocellum orotidine ′-phosphate decarboxylase gene; hpt, T. saccharolyticum hypoxanthine phosphoribosyltransferase gene.