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Table 3 Number of transformants isolated when varying Dcm methylation of plasmids with different origins of replication

From: Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum

  

Average number of transformants per µg DNA (range)

Plasmida

Replication origin

# Dcm sitesb

Top10 DNAc

BL21 DNAc

pMU1117

pMK3

11

1 (0−3)

660 (220−1300)

pMU1054

pB6A

9

0 (0)

2600 (780−5600)

  1. aPlasmid sizes: pMU1117, 9018 bp; pMU1054, 6833 bp.
  2. bNumber of E. coli Dcm methylation sites (CCWGG) present in the plasmid.
  3. cE. coli strain from which plasmid DNA was isolated.