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Table 3 Number of transformants isolated when varying Dcm methylation of plasmids with different origins of replication

From: Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum

   Average number of transformants per µg DNA (range)
Plasmida Replication origin # Dcm sitesb Top10 DNAc BL21 DNAc
pMU1117 pMK3 11 1 (0−3) 660 (220−1300)
pMU1054 pB6A 9 0 (0) 2600 (780−5600)
  1. aPlasmid sizes: pMU1117, 9018 bp; pMU1054, 6833 bp.
  2. bNumber of E. coli Dcm methylation sites (CCWGG) present in the plasmid.
  3. cE. coli strain from which plasmid DNA was isolated.