Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose

Table 2 Characteristics of glucose-tolerant β-glucosidases from T. thermosaccharolyticum DSM 571 and other microorganisms

Strain	K_m (mM)		V_max (U/mg)		K_i for glucose (mM)	Cellobiose inhibition (%)	K_cat/K_m (mM^-1 s^-1) for cellobiose	Optimal Temp (°C)
Strain	pNPG^a	Cellobiose	pNPG	Cellobiose	K_i for glucose (mM)	Cellobiose inhibition (%)	K_cat/K_m (mM^-1 s^-1) for cellobiose	Optimal Temp (°C)
T. thermosaccharolyticum	0.63	7.9	64	120	600	No effect	13.3	70
Uncultured bacterium [13]	0.39	20.4	50.7	15.5	1000	ND^b	0.65	40
Debaryomyces vanrijiae[18]	0.77	57.9	668	84.3	439	ND	2.43	40
A. oryzae[19]	0.55	7	1,066	353	1,390	50	36.1	50
A. niger[12]	21.7	ND	124.4	ND	543	ND	ND	55
A. tubingensis[10]	6.2	ND	28.4	0.32	600	ND	ND	60
Candida peltata[21]	2.3	66	108	8.5^c	1400	No effect	0.1^c	50
Scytalidium thermophilum[20]	0.29	1.61	13.27	4.12	>200	ND	1.7	60

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