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Table 2 Characteristics of glucose-tolerant β-glucosidases from T. thermosaccharolyticum DSM 571 and other microorganisms

From: Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose

Strain K m (mM) V max (U/mg) K i for glucose (mM) Cellobiose inhibition (%) K cat /K m (mM-1 s-1) for cellobiose Optimal Temp (°C)
pNPGa Cellobiose pNPG Cellobiose
T. thermosaccharolyticum 0.63 7.9 64 120 600 No effect 13.3 70
Uncultured bacterium [13] 0.39 20.4 50.7 15.5 1000 NDb 0.65 40
Debaryomyces vanrijiae[18] 0.77 57.9 668 84.3 439 ND 2.43 40
A. oryzae[19] 0.55 7 1,066 353 1,390 50 36.1 50
A. niger[12] 21.7 ND 124.4 ND 543 ND ND 55
A. tubingensis[10] 6.2 ND 28.4 0.32 600 ND ND 60
Candida peltata[21] 2.3 66 108 8.5c 1400 No effect 0.1c 50
Scytalidium thermophilum[20] 0.29 1.61 13.27 4.12 >200 ND 1.7 60
  1. a pNPG: p-nitrophenyl-β-D-glucopyranoside.
  2. b ND: not determined.
  3. c It was calculated by the data based on the reference.