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Table 2 Physiological characterization: Anaerobic fermentation in minimal medium with 20 g l-1 glucose and xylose, respectively

From: Evolutionary engineering strategies to enhance tolerance of xylose utilizing recombinant yeast to inhibitors derived from spruce biomass

Strain

μmax

YX/S

YE/S

Ygly/S

YAc/S

rglu

rxyl

rE

TMB3400

0.37±0.01

0.11±0.00

0.35±0.00

0.10±0.00

0.005±0.001

3.35±0.05

0.10±0.00

1.17±0.02

RK60-5

0.42±0.01

0.11±0.00

0.36±0.00

0.10±0.00

0.005±0.000

3.87±0.07

0.00

1.39±0.02

RKU90-3

0.44±0.00

0.11±0.00

0.37±0.00

0.11±0.01

0.005±0.001

4.15±0.02

0.00

1.54±0.02

KE1-5

0.34±0.01

0.11±0.00

0.36±0.01

0.10±0.01

0.007±0.001

3.03±0.05

0.05±0.00

1.08±0.01

KE1-17

0.41±0.01

0.12±0.01

0.36±0.01

0.09±0.00

0.007±0.000

3.56±0.06

0.08±0.02

1.26±0.01

  1. The maximum specific growth rate (μmax) is represented in h-1. The specific consumption rate of glucose (r glu), xylose (r xyl) and specific ethanol productivity (r E) are represented in g g cell dry weight-1 h-1. The yield of biomass (Y X/S), ethanol (Y E/S), glycerol (Y gly/S), and acetate (Y Ac/S) are represented in g g consumed sugars-1. All values are calculated during the exponential phase identified by natural logarithm of biomass and culture time. The values are average from duplicate experiments and ± indicates deviation from the mean.