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Table 3 Physiological characterization: Anaerobic fermentation in spruce hydrolysate

From: Evolutionary engineering strategies to enhance tolerance of xylose utilizing recombinant yeast to inhibitors derived from spruce biomass

Strain μmax YX/S YE/S Ygly/S rsugars* rE rHMF rfur
TMB3400 0.07±0.01 0.07±0.01 0.40±0.02 0.05±0.01 1.09±0.17 0.44±0.07 0.08±0.00 0.07±0.01
RK60-5 0.10±0.01 0.07±0.00 0.44±0.00 0.04±0.00 1.50±0.01 0.66±0.01 0.08±0.01 0.08±0.01
RKU90-3 0.10±0.01 0.07±0.01 0.43±0.01 0.04±0.01 1.49±0.01 0.63±0.01 0.07±0.00 0.08±0.00
KE1-5 0.07±0.00 0.07±0.01 0.44±0.02 0.03±0.01 1.07±0.20 0.47±0.11 0.08±0.01 0.07±0.01
KE1-17 0.08±0.00 0.06±0.00 0.43±0.00 0.02±0.00 1.36±0.09 0.58±0.03 0.09±0.01 0.11±0.00
  1. The maximum specific growth rate (μmax) is represented in h-1. The specific consumption rate of sugars (r sugars), specific conversion rate of HMF (r HMF), furfural (r fur) and specific ethanol productivity (r E) are represented in g g cell dry weight-1 h-1. The yield of biomass (Y X/S), ethanol (Y E/S) and glycerol (Y gly/S) are represented in g g consumed sugars-1. All values are calculated during the exponential phase identified by natural logarithm of biomass and culture time. The values are average from duplicate experiments and ± indicates deviation from the mean. *Specific consumption rate of glucose and mannose, since during the exponential phase glucose and mannose are co-consumed.