Figure 3

Hierarchical clustering of time course profiles during fermentation of glucose and xylose. The heat map was generated using either quantified metabolite concentrations or normalized areas of the chromatographic peaks (indicated by *) and shows the log₂ fold change relative to the first sampling point (t = 14.5 h) which represents exponential growth on a mixture of glucose and xylose. The hierarchical clustering of metabolite profiles yields four major clusters: Cluster A contains 15 metabolites which increase in either of the two strains; Cluster B contains 8 metabolites which show little change during the fermentation; Cluster C contains 12 metabolites which decrease moderately in at least one strain; Cluster D contains 9 metabolites which decrease markedly in both the XR/XDH-strain and the XI-strain.