Growth characterization of P. tricornutum. Cell density growth curve of P. tricornutum cells (▲) showing depletion of exogenous nitrate (○) and phosphate (◊). Phosphate concentrations are multiplied by a factor of 10 for visualization (A). Cell density growth curve showing the depletion and rebound of dissolved inorganic carbon (∆) throughout P. tricornutum growth (B). Arrows indicate time points at which cells were harvested for RNA sequencing analysis.