Differential expression of genes encoding enzymes with similar function in fatty acid and lipid biosynthesis. (A) Expression of ketoacyl ACP synthase (KAS) genes. KAS I, ketoacyl ACP synthase I; KAS II, ketoacyl ACP synthase II; KAS III, ketoacyl ACP synthase III. (B) Expression of lysophosphatidyl acyltransferase (LPAT) genes. LPAT1, Lysophosphatidyl acyltransferase 1; LPAT2, Lysophosphatidyl acyltransferase 2; LPAT4, Lysophosphatidyl acyltransferase 4; LPAT5, Lysophosphatidyl acyltransferase 5. (C) Expression of oleosin genes. (D) Expression of desaturase genes. FAD, fatty acid desaturase; FAD5, fatty acid desaturase 5; FAD6, fatty acid desaturase 6; AAD, acyl-ACP desaturase; DALD, δ-12-acyl-lipid desaturase; D7SD, δ-7-C-5 sterol desaturase; D9SD, δ-9-stearoyl-acyl carrier protein desaturase; D12FAC, δ-12 fatty acid conjugase; SD, sterol desaturase; FAD2, fatty acid desaturase 2. The gene transcripts were measured by qRT-PCR. Results are shown as the ratios of transcripts fatty acid and lipid biosynthetic genes over that of the Actin1 gene of jatropha. The experiments were performed in triplicate and the data are presented as means ± SD.