Table 1 Summary of fatty acid and lipid biosynthetic genes in this study

Ketoacyl ACP synthase I (KAS I)

I

TGGTTGGTCTTCTTTCCCTC

ACAAACCCACCAGCTTCATAG

JQ806261

Ketoacyl ACP synthase II (KAS II)

I

ACAGTCACCTTGTTTTTGTTCC

ATGCTAATTTACCCTGATAAGG

JQ806262

Ketoacyl ACP synthase III (KAS III)

I

CAATTATTAGATGGGGCTGAAG

TATGTGACAACAGAAACCAAGC

JQ806263

3-ketoacyl-CoA reductase isoform 1 (KCR1)

I

TCCTTTATTTGGGGTTTGTTAC

AGCAACCTAAAAGTTCAATTCC

JQ806264

3-ketoacyl-CoA reductase isoform 3 (KCR3)

I

ATGGACAGTAACATAGCCAATC

TCCACACACTTTTTCACAACTG

JQ806266

Acyl carrier protein1 (ACP1)

I

ACCGTTCAGGAAGCTGCTG

ACATAGTTCACAACAAGATTGC

JQ806272

Acyl-CoA dehydrogenase (ACD)

I

AATTGCAGATGGCCCCGATG

AGTTTTTGTGTGCTGGTATTGG

JQ806273

Semialdehyde decarboxylase 1 (SAD1)

I

GGGGCAGTGATTTTCCATTTG

GTGCTTTTTCCATATCTAATGAC

JQ806274

Hydroxyacyl-ACP dehydratase (HAD)

I

CTGCTATCCTATGCCTTTTTTG

GTTTTGCCCATAAGTTTAACATC

JQ806275

Acyl-ACP thioesterase (FATA)

I

TATTTGTGTGGGCATCTGCC

TAGTTGGTAAGGTGGGTTTAAC

JQ806276

Enoyl-ACP reductase (EAR)

I

ATGGGTGTGGGAGTTGACAG

GTGACATGGCATGGATTAAATG

JQ806277

Phosphatidic acid phosphatase β (β-PAP)

I

CACGAGCCCCATTCTGGAC

AGTCTGTTCAAGGTCAGGGG

JQ806281

CDP-diacylglycerol synthase (CDP-DAG)

I

CTAATAACAGTGTCATGGCAG

TTCCATATTCACTAAGTGCATTG

JQ806282

Digalactosyldiacylglycerol synthase 1 (DGD1)

I

AGACCTGCATCTCTACCTCC

GGTGCTGCCTAAATCTATATTC

JQ806283

Monogalactosyldiacylglycerol synthase (MGD2)

I

GTGTAAAGAATGGCAAGCATG

CCCCTAAAAGAATCAGAAACC

JQ806284

Phosphatidylinositol synthase (PTS)

I

CTTTTCATCTTCTGTGTCCATG

TAGTCAATAACCATCTCGTGC

JQ806287

Short-chain acyl-CoA oxidase (SCAOX)

I

AGGTTCTGCTTTTGCGCTAC

GGTCCCCTAGCTGGTAATTC

JQ806289

Long-chain acyl-CoA oxidase (LCAOX)

I

TGCACCAAGAGTATGATAGGC

TTACGTTTCTTTGTTCCAGCC

JQ806290

Acyl-CoA oxidase (AOX)

I

CCGTAATGCAAGACTGTGAAG

TTGCCATTAACTTGGATACAGC

JQ806291

Long-chain acyl-CoA synthetase (LACS)

I

CAAGAGAGAGGCCATCAGG

AAAATCCAAGAGAAACAGCAAG

JQ806292

Oleosin 3 (Oleosin 3)

I

AAGAGAAGTGGGTTTTGGTGG

AGAAACAAAAAGATTTAAGCG

JQ806304

Diacylglycerol kinase (DGK)

I

GTGGCTCAGATTTGGGTTGC

AAACTATTGAAGCTAAGCCTGG

JQ806306

D-erythro-sphingosine kinase/diacylglycerol kinase (DeDGK)

I

ATCAAATTCAGGAAAAGTAGCG

AATCAAACTGCACAAAAGGAAC

JQ806308

Calmodulin-binding diacylglycerol kinase (cDGK)

I

AGAAGATAAGGAAGAGCGAAG

GTTATAGCCTACAGCCAAAGC

JQ806309

Protein phosphatase 2C (PP2C)

I

TCATGGGCTTAAATGTGTGTAC

AACTCATACTTGAAAAGCTAAGG

JQ806311

Tyrosine phosphatase (TP)

I

TGGCCGTTTGTTAAG ATTGATC

ATTGACTTCATAATGTTGACCC

JQ806312

Phosphoglycolate phosphatase (PGP)

I

ATGCTGATGGGCTTTACTTTG

AGAACTAGCAAACTCCTTCCC

JQ806314

Lysophosphatidyl acyltransferase 1(LPAT1)

I

CTGAAGGTTAGTGCAACAAATG

GTAACATCGTCTGGAAAATTGC

JQ806317

Lysophosphatidyl acyltransferase 2 (LPAT2)

I

CTTTGGTTTCATGTGCTGCAC

ATACATGAAAAGAAAAGGTGCC

JQ806320

Lysophosphatidyl acyltransferase 5 (LPAT5)

I

TTTGGCATCTGCAACCTATTTC

GCCATAAACAGGTATGAGTCTC

JQ806319

Diacylglycerol acyltransferase (DGAT1)

I

GACCTAATGAATCGGAAAGGC

CCGCATAGCCAAAATTGCTTG

JQ806316

Phospholipid/glycerol acyltransferase (PL/GA)

I

TAAAGTATTCTCGCCCTAGCCC

ACATTTGCTTCTGTTTTCATGC

JQ806322

Triacylglycerol lipase (TAGL)

I

GTGAATACTGTTGTAAGCCTG

GTCCAAAAACACCAATGAAATG

JQ806324

Phospholipase C (PLC)

I

CAGCTCAATGGTGATTATGTC

AGCTTTTATGTAATTTGCGTCG

JQ806325

Phospholipase D (PLD)

I

ACTATGGGCAGAGCATGTTG

TCACATCCAGGAATAGCCTC

JQ806326

Phospholipase D α (PLDα)

I

CGCCAAATCTGATTACCTCC

CACTGATATGAACATCCTGGC

JQ806327

Phosphatidylinositol 4-kinase (P4K)

I

AAGACTTCTAGGGTTTGTGGG

CTCCTCAGTCCTCACTTAGC

JQ806329

Putative phosphatase (PP)

I

TGGGAAGATGCCATGTCTATC

CCAAACAAGAGATAAACTAACAG

JQ806330

Fatty acid desaturase (FAD)

I

ATGACCAATCCTGTTCCAAAG

TGCTAATGTTTACAAATGAGGG

JQ806294

Fatty acid desaturase 5 (FAD5)

I

ACTTGGTATGTTGTGAGGTTTC

ATGTAGAAAAGCTAATGCCCC

JQ806295

Fatty acid desaturase 6 (FAD6)

I

TTGCCCCTGAAGAATCTCAAC

ATTCATATTACTGTCCTCCCC

JQ806296

Acyl-ACP desaturase (AAD)

I

AGTTTTTGATCGGACGGTGG

AAAGAGAAGAAAGCAAGACTCG

JQ806300

δ-12-acyl-lipid desaturase (DALD)

I

GCTATAATATGTGGTTTGGCC

GTTGTAGAGTTCCATAAACGG

JQ806301

δ-7-C-5 sterol desaturase (D7SD)

I

TCTTGCATAGGCATCCATTTC

AGATCGAGTACATGGCTATGG

JQ806302

δ-9-stearoyl-acyl carrier protein desaturase (D9SD)

I

TGTTTGGAGAAGACATACCGG

CAGGGCTGTGGTGACTTAC

JQ806303

δ-12 fatty acid conjugase (D12FAC)

I

AGCCAGAAGAGGGAGGTCC

AACTCAAACACCACTTTCCCG

JQ806298

Fatty acid desaturase 2 (FAD2)

I

TGGACTACTTGTTAGGAATTTG

GTTCATATTGTTTCTACCTGGG

JQ806297

Sterol desaturase (SD)

II

CTGATGAAGGCACACTGTTG

GTAGAAACATAATCCACTGCC

JQ806299

3-ketoacyl-CoA reductase isoform 2 (KCR2)

II

CGTCTCTCTCTCGGAATCC

TCTTCTGTGAAAACGACCCTC

JQ806265

Enoyl-CoA hydratase (ECH)

II

AGATTGGAGGAATGGTCAAAG

TATTGCTTGCTAGGATTGGAG

JQ806267

Oleosin (Oleosin)

II

TGGACAGTATATGCAACACAAG

TATTCCACACTGAAATTAGCAC

JQ806305

Long-chain acyl-CoA synthetase 8 (LACS8)

II

TTTGTTTAATGTGCTTTCCTCC

GTCCTGCAATTTAGGTGAAGC

JQ806293

Diacylglycerol kinase 1 (DGK1)

II

TGGCACTTAGGCTGACTTAG

CAGCTAAAAGCACCAAGTTAAG

JQ806307

Lysophosphatidyl acyltransferase 4 (LPAT4)

II

ATTTTAGCATGTGCATTCCTTG

TATAAACAAGTTCACAAAAAGGTC

JQ806318

Lipase (Lipase)

II

TGGGGTTCAATGCCAAAGAC

TAGCCTGTCTACAGATTTTCC

JQ806323

Ketoacyl-CoA synthase (KCS)

III

CTTTGATTTGTACTTTCATGGG

AACACACAAGCATTTGAAGCC

JQ806268

Choline kinase (CLK)

III

TATTTCTTCCTGCGATACAATG

TTTGGATCTTAAATCTGGCTAC

JQ806313

Protein phosphatase 2A (PP2A)

IV

TGATGTTACCCGTAGAACTCC

ATAAAAAAAAACAAAACCTGCCC

JQ806310

Ketoacyl ACP reductase (KAR)

IV

CGGAAGAGGTTGCAGGATTG

AAAAACTGCCTCAACACAAGC

JQ806278

Phosphatidic acid phosphatase α (α-PAP)

IV

ATGGGGCTATATTTGGCTCAC

CTTGGAAACCTGATAAACAAAAG

JQ806280

Cholinephosphate cytidylyltransferase (CPC)

IV

GACAAAGATGATGCTAAGGAG

TATTCCTATCCTCACAACAAGC

JQ806285

Cyclopropane-fatty-acyl-phospholipid synthase (CFAS)

IV

GACTTGTCTTCCTGAGAGCC

GCAGTTCTTTGAAAGCGATGG

JQ806286

Sulfoquinovosyldiacylglycerol synthase type 2 (SQD2)

IV

GCAGCCACTAGAAAAATCCG

GCAGAGCAAATCCGTCACC

JQ806288

Phosphotyrosine protein phosphatase (PPP)

IV

ATGTGCTCCTTTTGTAAGAAAC

AACTTTGAATGCCGCTGGTC

JQ806315

Glycerol-3-phosphate acyltransferase (G3PAT)

IV

GGCAGTAATGTCTTTGGTTTC

TACATGAAAAGAAAGGGTGCC

JQ806321

Phosphatidylinositol 3-kinase (P3K)

IV

TGGAGTTTGCTCAAGGAGTC

TTAAAAAAGAGCTGAAAGACACC

JQ806328

Malonyl CoA ACP transacylase (MCAT)

V

GTTATTGCTGGCATTGTCAAG

GAAATCTCTAGTACATGACGC

JQ806279

Lipid phosphate phosphatase 3 (LPP3)

V

TCTGAGACGAGCGGAGGAC

ATTCATCATCTCCTTCCAGTTC

JQ806270

Jatropha Actin 1 protein (Actin1)

Control

TAATGGTCCCTCTGGATGTG

AGAAAAGAAAAGAAAAAAGCAGC

JQ806331