Figure 3

Glycome profiling of Sorghum bicolor near-isogenic lines before and after inoculation with Clostridium phytofermentans. Sequential extractions using (A) oxalate, (B) carbonate, and (C) 1 mol/l and (D) 4 mol/l potassium hydroxide of five replicates of inoculated and uninoculated wild type, bmr-6, bmr-12, and bmr-6/bmr-12 sorghum were generated as described previously [29]. ELISAs using 147 monoclonal antibodies directed against glycan were used to detect the presence of cell-wall glycan epitopes in each extraction fraction, and the data are presented as heat maps (see Additional file 1; see Additional file 2). Plant biomass samples tested are indicated at the top of each column. Antibodies are grouped according to the recognized glycan are listed on the right panel. The black-red-white scale indicates the strength of the ELISA signal: bright-red, white, and dark-blue colors depict strong, medium, and no binding, respectively. AG, arabinogalactan; FUC-XG, fucosylated xyloglucan; HG, homogalacturonan; RG, rhamnogalacturonan.