Figure 4

Polysaccharide conversion by Clostridium pytofermentans of wild type and brown midrib mutant sorghum. The remaining biomass pellet was given sequential extractions using (A) oxalate, (B) carbonate, and 1 mol/l and 4 mol/l potassium hydroxide followed by glycome profiling [29]. For each genotype, values represent the difference in extractable xylan between the mean ± SEM of five uninoculated samples and each of the inoculated samples. Xylan-3a (CCRCM-137, -149, -160), -3b (CCRCM-144, -146, -152, -155), and -4 (CCRCM-138, -139, -140, -148, -151, -153) are groups of antibodies generated using either oat or corncob xylan as an immunogen, and are classified by hierarchical clustering analysis of their recognition patterns. Data are mean ± SEM of five replicates; bars annotated with the same letter are not significantly different at P < 0.05 (Scheffé's test for multiple comparisons).