TY - JOUR AU - Gourlay, Keith AU - Arantes, Valdeir AU - Saddler, Jack N. PY - 2012 DA - 2012/07/24 TI - Use of substructure-specific carbohydrate binding modules to track changes in cellulose accessibility and surface morphology during the amorphogenesis step of enzymatic hydrolysis JO - Biotechnology for Biofuels SP - 51 VL - 5 IS - 1 AB - Cellulose amorphogenesis, described as the non-hydrolytic “opening up” or disruption of a cellulosic substrate, is becoming increasingly recognized as one of the key steps in the enzymatic deconstruction of cellulosic biomass when used as a feedstock for fuels and chemicals production. Although this process is thought to play a major role in facilitating hydrolysis, the lack of quantitative techniques capable of accurately describing the molecular-level changes occurring in the substrate during amorphogenesis has hindered our understanding of this process. SN - 1754-6834 UR - https://doi.org/10.1186/1754-6834-5-51 DO - 10.1186/1754-6834-5-51 ID - Gourlay2012 ER -