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Table 1 Non-hydrolytic disruptive proteins and their effects on biomass

From: Use of substructure-specific carbohydrate binding modules to track changes in cellulose accessibility and surface morphology during the amorphogenesis step of enzymatic hydrolysis

Proteins with unknown catalytic mechanism Putative function References
Family 1 and 2 CBMs Fibre pitting/roughening, small particle release [18, 19, 23, 30]
Swollenin, Loosenin Fibre swelling, microfibril dispersion, dispersion of cellulose aggregates [16, 17, 27]
Expansins Loosening of plant cell walls, solubilization of oligomeric sugars [21, 31, 39]
Expansin-like proteins Loosening of filter paper, dispersion of cellulose aggregates [25, 78]
Fibril Forming Protein Fibril release from filter paper [15]
Proteins with putative oxidative catalytic mechanism Putative function References
GH61 Oxidative cleavage of crystalline cellulose [3336]
CBM33 Oxidative cleavage of crystalline cellulose [37, 38]
  1. The non-hydrolytic proteins capable of promoting amorphogenesis of cellulosic and/or lignocellulosic biomass identified to date. Amorphogenesis induced by these proteins has been shown to manifest as a range of specific disruptive effects.