Use of substructure-specific carbohydrate binding modules to track changes in cellulose accessibility and surface morphology during the amorphogenesis step of enzymatic hydrolysis

Table 1 Non-hydrolytic disruptive proteins and their effects on biomass

Proteins with unknown catalytic mechanism	Putative function	References
Family 1 and 2 CBMs	Fibre pitting/roughening, small particle release	[18, 19, 23, 30]
Swollenin, Loosenin	Fibre swelling, microfibril dispersion, dispersion of cellulose aggregates	[16, 17, 27]
Expansins	Loosening of plant cell walls, solubilization of oligomeric sugars	[21, 31, 39]
Expansin-like proteins	Loosening of filter paper, dispersion of cellulose aggregates	[25, 78]
Fibril Forming Protein	Fibril release from filter paper	[15]
Proteins with putative oxidative catalytic mechanism	Putative function	References
GH61	Oxidative cleavage of crystalline cellulose	[33–36]
CBM33	Oxidative cleavage of crystalline cellulose	[37, 38]

The non-hydrolytic proteins capable of promoting amorphogenesis of cellulosic and/or lignocellulosic biomass identified to date. Amorphogenesis induced by these proteins has been shown to manifest as a range of specific disruptive effects.

ISSN: 2731-3654