A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover

Table 1 Identified hemicellulose-degrading proteins and spectrum counts on 1, 3, 7, and 14 days

				Spectrum count^c
Accession number^a	GH family^a	Identified proteins^a	MW (kDa)^b	Day 1	Day 3	Day 7	Day 14	SignalP^d
AN8401	GH3	Beta-1,4-xylosidase	82	60	98	107	132	Y
AN2217	GH3	Beta 1,4-Xylosidase	83	39	48	58	79	Y
AN2359	GH3	Beta-xylosidase	87	53	115	57	0	Y
AN1818	GH10	Beta-1,4-endoxylanase	34	101	142	576	720	Y
AN7401	GH10	Beta-1,4-endoxylanase	38	0	4	11	31	Y
AN3613	GH11	Beta-1,4-endoxylanase A precursor	24	188	174	194	140	Y
AN7152	GH27	Alpha-1,4-galactosidase	69	67	138	124	121	Y
AN8138	GH36	Alpha-1,4-galactosidase	82	0	0	27	24	Y
AN7117	GH39	Xylosidase	50	0	9	13	12	Y
AN8007	GH43	Endoarabinase	34	6	29	20	19	Y
AN2533	GH43	Alpha N-arabinofuranosidase	36	0	13	10	7	Y
AN7781	GH43	Arabinosidase, putative	38	32	74	52	60	Y
AN2534	GH43	Endoarabinase	41	0	13	12	7	Y
AN10919	GH43	1,4-endoxylanase D precursor	42	2	39	50	48	Y
AN7313	GH43	Alpha L-arabinofuranosidase C	52	0	5	0	0	Y
AN7275	GH43	Putative xylosidase	55	0	0	0	24	Y^e
AN8477	GH43	Xylosidase/arabinofuranosidase	60	37	69	64	97	N^f
AN5727	GH53	Beta-1,4-endogalactanase	41	11	19	16	18	Y
AN1571	GH54	Alpha-arabinofuranosidase	53	45	98	80	96	Y
AN2632	GH62	Arabinoxylan/arabinofuranohydrolase	33	13	30	29	21	Y
AN7908	GH62	Arabinoxylan/arabinofuranohydrolase	36	27	106	90	113	Y
AN9286	GH67	Alpha-glucuronidase	94	14	17	69	104	Y
AN5061	GH74	Xyloglucanase	88	0	0	0	7	Y
AN2060	GH93	Exo-arabinanase	43	17	24	24	27	Y
AN6093	CE1	Acetyl xylan esterase	34	0	9	6	4	Y
AN1320		Beta-1,4-endoxylanase B	28	10	36	46	55	Y
AN6673		Alpha-fucosidase	92	-	-	30	31	Y
AN9380		Bifunctional xylanase/ deacetylase	26	10	6	9	14	Y

^aAccession numbers along with protein information and glycosyl hydrolase (GH) family information was obtained from Pedant [23].
^bHypothetical molecular weight of the proteins.
^cQuantifying changes in protein abundance between samples from different time points was done using the spectral count method, yielding a semiquantitative analysis.
^dSignalP was used to predict secretion signals [23, 24].
^eSignalP as reported at [25].
^fNot found by SignalP (N-terminal may be incorrectly annotated , a novel signal peptide may be present, or the protein is normally intracellular but was released by autolysis).

ISSN: 2731-3654