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Table 10 Identified esterases and lipases and spectrum counts on 1, 3, 7, and 14 days

From: A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover

  

Spectrum countc

 

Accession numbera

GH familya

Identified proteinsa

MW (kDa)b

Day 1

Day 3

Day 7

Day 14

SignalPd

AN5309

CE5

Putative cutinase

22

17

15

39

48

Y

AN7541

CE5

Cutinase, putative

26

23

0

0

0

Y

AN2834

CE12

Esterase, putative

27

6

0

0

0

Y

AN6422

CE16

Cellulose-binding GDSL Lipase/Acylhydrolase

33

0

4

10

9

Y

AN5321

 

Extracellular lipase putative

62

75

103

71

50

Y

AN9287

 

GDSL lipase/acylhydrolase

47

23

52

49

50

Y

AN7691

 

Phosphoesterase superfamily protein

50

23

55

44

13

Y

AN1792

 

GDSL lipase/acylhydrolase

38

16

54

35

37

Y

AN8046

 

Putative extracellular lipase

31

11

48

44

47

Y

AN1433

 

Putative triacylglycerol lipase

60

24

16

6

0

Y

AN1799

 

Putative lipase

48

0

0

13

39

Y

AN7046

 

Similar to triacylglycerol lipase

25

3

0

0

0

Y

AN9361

 

Lipase

63

5

-

-

-

Y

  1. aAccession numbers along with protein information and glycosyl hydrolase (GH) family information was obtained from Pedant [23].
  2. bHypothetical molecular weight of the proteins.
  3. cQuantifying changes in protein abundance between samples from different time points was done using the spectral count method, yielding a semiquantitative analysis.
  4. dSignalP was used to predict secretion signals [23, 24].