A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover

Table 3 Identified pectin-degrading proteins and spectrum counts on 1, 3, 7, and 14 days

				Spectrum count^c
Accession number^a	GH family^a	Identified proteins^a	MW (kDa)^b	Day 1	Day 3	Day 7	Day 14	SignalP^d
AN2463	GH2	Beta-galactosidase	115	0	0	50	96	N^f
AN2395	GH2	Beta-galactosidase/mannosidase	69	25	70	83	81	Y
AN8761	GH28	Exopolygalaturonase	48	49	38	18	0	Y
AN8891	GH28	Exopolygalaturonase	49	30	20	0	0	Y
AN10274	GH28	Exo-polygalacturonase, putative	46	0	4	0	0	Y
AN0980	GH35	Beta-galactosidase	109	2	14	8	25	Y
AN0756	GH35	Beta-galactosidase	109	0	5	2	8	Y
AN7151	GH78	Alpha-rhamnosidase	100	4	14	64	83	N^f
AN7828	GH88	Unsaturated rhamnogalacturonan hydrolase	44	11	0	0	0	Y
AN9383	GH105	Unsaturated rhamnogalacturonan hydrolase	43	92	54	60	39	Y
AN0741	PL1	Pectate lyase precursor	35	7	41	28	41	Y
AN2331	PL1	Pectin lyase A precursor	41	17	0	0	0	Y
AN2569	PL1	Pectin lyase A precursor	39	32	29	47	31	Y
AN7646	PL1	Pectate lyase A	35	4	3	19	18	Y
AN6106	PL3	Pectate lyase C	26	6	22	20	23	Y
AN8453	PL3	Pectate lyase C	28	10	0	5	3	Y
AN7135	PL4	Rhamnogalaturonan lyase	56	13	71	71	80	Y
AN4139	PL4	Rhamnogalaturonan lyase	117	6	15	3	5	Y
AN3390	CE8	Pectin methylesterase	35	0	19	11	16	Y
AN4860	CE8	Pectin methylesterase	42	27	3	0	0	Y
AN2528	CE12	Rhamnogalaturonan acetyl esterase	26	4	0	16	16	Y
AN2537		Exopolygalacturonate lyase	44	4	12	6	5	Y

^aAccession numbers along with protein information and glycosyl hydrolase (GH) family information was obtained from Pedant[23].
^bHypothetical molecular weight of the proteins.
^cQuantifying changes in protein abundance between samples from different time points was done using the spectral count method, yielding a semiquantitative analysis.
^dSignalP was used to predict secretion signals [23, 24].
^eSignalP as reported at [25].
^fNot found by SignalP (N-terminal may be incorrectly annotated , a novel signal peptide may be present, or the protein is normally intracellular but was released by autolysis).

ISSN: 2731-3654