A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover

Table 7 Identified mannan degrading proteins and spectrum counts on 1, 3, 7, and 14 days

				Spectrum count^c
Accession number^a	GH family^a	Identified proteins^a	MW (kDa)^b	Day 1	Day 3	Day 7	Day 14	SignalP^d
AN5361	GH2	Beta-1,4-mannosidase	71	0	3	0	0	Y
AN9276	GH5	Beta-1,4-endomannanase	42	0	5	8	15	Y
AN6427	GH5	Beta-1,4-endomannanase	45	0	0	4	6	Y
AN2936	GH38	Alpha-mannosidase	124	0	4	38	72	N^f
AN0787	GH47	Similar to class I alpha-mannosidase 1B	56	10	48	53	58	Y
AN2325	GH92	Alpha-1,2-mannosidase	82	0	11	15	7	Y
AN1197	GH92	Alpha-1,2-mannosidase	88	0	3	7	6	Y

^aAccession numbers along with protein information and glycosyl hydrolase (GH) family information was obtained from Pedant [23, 24].
^bHypothetical molecular weight of the proteins.
^cQuantifying changes in protein abundance between samples from different time points was done using the spectral count method, yielding a semiquantitative analysis.
^dSignalP was used to predict secretion signals [23, 24].
^eSignalP as reported at [25].
^fNot found by SignalP (N-terminal may be incorrectly annotated , a novel signal peptide may be present, or the protein is normally intracellular but was released by autolysis).

ISSN: 2731-3654