Figure 1

Genomic integration of five gene cassettes into KR3. (A) Each of the five gene cassettes contains an independent promoter, alpha factor from K. lactis, a gene coding region, a terminator, and a 46 bp fragment homologous to its neighboring cassette. The KanMX, EGIII, CBHI, GFP, and NpaBGS gene cassettes are shown in orange, blue, dark blue, green, and purple, respectively. (B) The gene cassettes assembled in the predesignated order, Kan-EGIII-CBHI-GFP-NpaBGS. (C) Fluorescence microscopy photograph of the genetically engineered strain, KR5.