Figure 4

Cellulolytic enzyme assays of K. marxianus transformants. The relative activities were conducted using (A) MUC, (B) Dye-CMC, (C) PASC, and (D) pNPG as the substrate, respectively. The commercial cellulolytic enzyme mixture kits with 0.5 unitCelluclast 1.5 L and 1 unit Novozyme 188 were used as benchmarks. The protein concentration of the supernatant of the K. marxianus transformants cultures were 1.3 mg/ml. *: P < 0.05 (significant), **: P < 0.01; ***: P < 0.001; N.S., non-significant.