Figure 4
Conversion of glucan to glucose and cellobiose using culture supernatants from AFEX-SG grown T. aurantiacus and Cellic CTec2 on various pretreated biomass substrates. Reaction conditions were: (2% w/v) biomass, 60°C, pH 5 for 72 hours with mixing. Switchgrass substrates were pretreated in three different ways: IL – ionic liquid, [C2mim][OAc]; DA – dilute acid, H2SO4; AFEX-, NH4. The saccharification reactions were performed at two different enzyme loadings, corresponding to recommended high and low enzyme loadings for Cellic Ctec2 (http://www.bioenergy.novozymes.com/). Equal amounts of protein from both T. aurantiacus supernatants and Cellic CTec2 were added to each reaction (g enzyme/g glucan): AFEX-high/low ∼ 0.065/0.016; IL-high/low ∼ 0.046/0.011; DA-high/low ∼ 0.043/0.010. Three replicates were performed and the error bars represent standard deviation from the mean.