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Table 2 Secreted cellulase and hemicellulase activities (U/mg or mU/mg) from thermophilic fungi grown on complex biomass substrates 1,2,3

From: Thermoascus aurantiacus is a promising source of enzymes for biomass deconstruction under thermophilic conditions

Strain/Carbon source

CMC (U/mg)

pNPC (mU/mg)

pNPG (mU/mg)

Xylan (U/mg)

pNPX (mU/mg)

T. aurantiacus

Switchgrass

0.17 ± 0.03

34.4 ± 1.31

177.3 ± 2.71

0.67 ± 0.01

8.8 ± 0.81

AFEX Switchgrass

0.04 ± 0.01

25.7 ± 0.40

45.6 ± 1.71

0.10 ± 0.02

12.4 ± 1.63

IL Switchgrass

0.02 ± 0.01

9.8 ± 0.26

54.8 ± 0.43

0.35 ± 0.01

10.3 ± 1.09

Microcrystalline Cellulose

1.94 ± 0.22

12.5 ± 1.60

93.8 ± 12.86

1.90 ± 0.62

6.92 ± 0.49

T. terrestris

Switchgrass

0.79 ± 0.02

11.3 ± 0.62

26.1 ± 1.14

2.44 ± 0.38

5.7 ± 0.47

AFEX Switchgrass

0.24 ± 0.01

3.7 ± 0.07

5.7 ± 1.21

0.66 ± 0.05

0.8 ± 0.37

IL Switchgrass

0.23 ± 0.02

7.8 ± 0.25

5.7 ± 0.23

1.02 ± 0.05

3.8 ± 0.41

Microcrystalline Cellulose

1.24 ± 0.23

16.2 ± 4.38

6.9 ± 1.76

3.27 ± 1.12

4.17 ± 1.27

  1. 1Specific activities based on protein concentrations obtained using the Bradford method.
  2. 2 Three replicates were performed and the error bars represent standard deviation from the mean.
  3. 3 Activities represented by each substrate is described in the Methods section.