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Table 2 Secreted cellulase and hemicellulase activities (U/mg or mU/mg) from thermophilic fungi grown on complex biomass substrates 1,2,3

From: Thermoascus aurantiacus is a promising source of enzymes for biomass deconstruction under thermophilic conditions

Strain/Carbon source CMC (U/mg) pNPC (mU/mg) pNPG (mU/mg) Xylan (U/mg) pNPX (mU/mg)
T. aurantiacus
Switchgrass 0.17 ± 0.03 34.4 ± 1.31 177.3 ± 2.71 0.67 ± 0.01 8.8 ± 0.81
AFEX Switchgrass 0.04 ± 0.01 25.7 ± 0.40 45.6 ± 1.71 0.10 ± 0.02 12.4 ± 1.63
IL Switchgrass 0.02 ± 0.01 9.8 ± 0.26 54.8 ± 0.43 0.35 ± 0.01 10.3 ± 1.09
Microcrystalline Cellulose 1.94 ± 0.22 12.5 ± 1.60 93.8 ± 12.86 1.90 ± 0.62 6.92 ± 0.49
T. terrestris
Switchgrass 0.79 ± 0.02 11.3 ± 0.62 26.1 ± 1.14 2.44 ± 0.38 5.7 ± 0.47
AFEX Switchgrass 0.24 ± 0.01 3.7 ± 0.07 5.7 ± 1.21 0.66 ± 0.05 0.8 ± 0.37
IL Switchgrass 0.23 ± 0.02 7.8 ± 0.25 5.7 ± 0.23 1.02 ± 0.05 3.8 ± 0.41
Microcrystalline Cellulose 1.24 ± 0.23 16.2 ± 4.38 6.9 ± 1.76 3.27 ± 1.12 4.17 ± 1.27
  1. 1Specific activities based on protein concentrations obtained using the Bradford method.
  2. 2 Three replicates were performed and the error bars represent standard deviation from the mean.
  3. 3 Activities represented by each substrate is described in the Methods section.