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Table 3 Mass spectrometry analysis of major protein bands isolated from T. aurantiacus AFEX-SG secretome.

From: Thermoascus aurantiacus is a promising source of enzymes for biomass deconstruction under thermophilic conditions

Prominent band

Description

Predicted MW

Peptides (95%)1

% Sequence coverage (95)2

∼50 kDa

Possible GH 7 Cellobiohydrolase [GenBank: CAD79781]

48 kDa

250

72

∼35 kDa

GH 5 Endoglucanase [GenBank: CAZ67882]/GH10 Xylanase [GenBank: CAB65468]

37 kDa/36 kDa

249/176

72/65

∼25 kDa

GH 61 [PDB: 3ZUD_A]

24 kDa

60

93

  1. 1 Number of unique peptides matching protein with 95% confidence.
  2. 2 Sequence coverage of identified protein with unique proteins matching with 95% confidence.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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