Rapid selection and identification of Miscanthus genotypes with enhanced glucan and xylan yields from hydrothermal pretreatment followed by enzymatic hydrolysis

Table 2 Comparison of Miscanthus and other selected biomass feedstocks

Biomass	Composition (% dry weight raw)		Theoretical EtOH yield (gal/dry ton feedstock)*	Pretreatment condition	Enzymatic hydrolysis	Sugar yield **
Biomass	Glu	Xyl	Theoretical EtOH yield (gal/dry ton feedstock)*	Pretreatment condition	Enzymatic hydrolysis	Glu	Xyl	Total
Miscanthus x giganteus cv. Illinois in this study	42.9	22.0	113	180°C, 10% solid loading, 35 min, logR₀ 3.90	60 FPU Spezyme CP plus 120 CBU Novozym 188 per gram glucan + xylan, 72 h	92	74	55.7
Corn stover [24]	36.1	21.4	100.3	210°C, 50% solid loading, 6 min, logR₀ 4.02	60 FPU Spezyme CP plus 120 CBU Novozym 188 per gram glucan + xylan, 72 h	61.1	87.6	40.8
Wheat straw [25]	37.8	22.8	105.6	188°C, 10% solid loading, 40 min, logR₀ 4.19	15 FPU celluclast plus 15 IU Novozym 188 per gram dry substrate, 72 h	91	80	52.6
Switchgrass [26]	32.2	20.3	91.5	190°C, 5% solid loading, 10 min, logR₀ 3.64	15 FPU cellulase per gram glucan, 72 h	N/A	73.1	N/A
Hybrid Poplar [27]	44	15	102.5	200°C, 15% solid loading, 10 min, logR₀ 3.94	40 FPU Spezyme CP plus 40 CBU Novozym 188 per gram glucan, 48 h	63.3	85.3	45.5

Glu-glucan, xyl-xylan, lig-lignin.
*Theoretical EtOH yields were calculated by NREL Theoretical Ethanol Yield Calculator [38].
** Sugar yields for glu and xyl were %, the totals of glu and xyl were g/100 dry weight.

ISSN: 2731-3654