Hemicelluloses negatively affect lignocellulose crystallinity for high biomass digestibility under NaOH and H2SO4 pretreatments in Miscanthus

Table 1 Cell wall composition and lignocellulose crystalline index in Miscanthus samples

Pair	Sample	Cell wall composition (% Dry matter)						CrI^&
Pair	Sample	Cellulose		Hemicelluloses		Lignin		Raw material
I	Mlu26 (H) ^§	29.86 ± 1.47	5%^@	25.84 ± 0.73**	25%	25.10 ± 0.11	−4%	39.15	−28%
I	Msi34 (L)	28.30 ± 0.58	5%^@	20.04 ± 0.44	25%	26.19 ± 0.41	−4%	51.77	−28%
II	Mfl03 (H)	26.85 ± 0.71**	−29%	19.57 ± 0.27	−3%	24.91 ± 1.40	−6%	46.26	−19%
II	Mlu01 (L)	35.88 ± 1.62	−29%	20.09 ± 0.42	−3%	26.36 ± 0.49	−6%	55.9	−19%
III	Mfl40 (H)	30.66 ± 0.55	−7%	22.31 ± 0.16**	15%	21.99 ± 0.30**	−31%	45.79	−27%
III	Msa02 (L)	32.96 ± 2.52	−7%	19.28 ± 0.46	15%	29.95 ± 0.63	−31%	59.83	−27%
IV	Msi56 (H)	36.70 ± 0.64**	35%	27.00 ± 0.57**	39%	24.40 ± 0.33	2%	38.92	−21%
IV	Mfl04 (L)	25.81 ± 0.63	35%	18.12 ± 0.20	39%	23.89 ± 0.52	2%	47.86	−21%
V	Msa20 (H)	27.10 ± 1.22	−1%	24.70 ± 0.32**	25%	27.38 ± 0.39**	22%	46.13	4%
V	Mfl08 (L)	27.27 ± 0.44	−1%	19.12 ± 0.11	25%	21.90 ± 0.54	22%	44.48	4%
VI	Mfl27 (H)	27.86 ± 0.70**	−31%	19.22 ± 0.65*	−10%	20.49 ± 0.64**	−37%	33.84	−31%
	Mlu12 (L)	38.07 ± 0.37		21.16 ± 0.02		29.80 ± 0.29		46.29

* and ** A significant difference at pair by t-test at p < 0.05 and 0.01 (n = 3); @ Percentage of the increased or decreased level at pair: subtraction of two samples divided by means of two values at pair; & CrI method was detected at ±0.05 ~ 0.15 (n = 5); § (H) or (L) Indicated the sample in the pair with high (H) or low (L) biomass digestibility.

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