Epigallocatechin gallate incorporation into lignin enhances the alkaline delignification and enzymatic saccharification of cell walls

Table 2 Proportion of lignin removed and recovery of alkali-insoluble residues and cell wall sugars from cell wall dehydrogenation polymers (CWDHPs)

CWDHP	Alkaline pretreatment (°C)	Lignin extracted (%)	Recovered (%)
			Residue	Glucose	Xylose	Arabinose	Galactose	Total sugars
Control	70	43.7d	70.4a	76.0a	69.7a	72.2a	77.8a	74.2a
	100	49.7d	57.6b	66.1b	56.7b	59.1b	56.2b	61.6b
	130	61.9c	45.6c	55.2c	51.1c	51.7c	35.2c	51.5c
EGCG	70	72.4b	56.7b	76.4a	57.5b	52.7c	54.5b	64.9b
	100	87.1a	36.3d	52.1c	31.2d	28.0d	35.2c	40.7d
	130	92.4a	25.6e	41.2d	21.6e	17.2e	15.3d	29.0e
Analysis of variance
CWDHP		*	***	***	***	***	***	***
Pretreatment		***	***	***	***	***	***	***
CWDHP X Pretreatment Interaction		NS	*	**	***	***	NS	**

The CWDHP were formed with monolignols (control) or with monolignols plus epigallocatechin gallate (EGCG) and pretreated with 0.075 M sodium hydroxide (15% w/w loading) at various temperatures.
^a Means within columns with unlike letters differ (P < 0.05).
NS not significant; * P < 0.05; ** P < 0.01, *** P < 0.001.

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