Epigallocatechin gallate incorporation into lignin enhances the alkaline delignification and enzymatic saccharification of cell walls

Table 3 Enzymatic and alkali-soluble (AS) glucose and total sugars released from cell wall dehydrogenation polymers (CWDHPs)

CWDHP	Alkaline pretreatment (°C)	Enzymatic (%)¹		Enzymatic (%)²		Enzymatic + AS (%)²
		Glucose	Total sugars	Glucose	Total sugars	Glucose	Total sugars
Control	None	37.9d	26.4d	37.9c	26.4bc	37.9e	26.4d
	70	60.7c	45.9c	46.1b	34.1ab	70.2d	59.9c
	100	66.6bc	52.3bc	44.0bc	32.2ab	77.9c	70.6b
	130	73.0b	54.9bc	40.3bc	28.3abc	85.1b	76.8b
EGCG	None	42.5d	30.2d	42.5bc	30.2abc	42.5e	30.2d
	70	72.9b	56.1b	55.6a	36.5a	79.3bc	71.6b
	100	86.3a	67.3a	44.9bc	27.4bc	92.9a	86.7a
	130	92.4a	76.2a	38.0c	22.1c	96.9a	93.1a
Analysis of variance
CWDHP		***	*	NS	NS	***	*
Pretreatment		***	**	*	NS	***	***
CWDHP X Pretreatment Interaction		*	*	*	***	†	*

The CWDHPs were formed with monolignols (control) or with monolignols plus epigallocatechin gallate (EGCG) and pretreated with 0.075 M sodium hydroxide (15% w/w loading) at various temperatures. Data are averaged over enzymatic hydrolyses conducted for 6, 12, and 24 h.
¹ Based on the concentration of glucose or total sugars in CWDHPs or their alkaline insoluble residues.
² Based on the concentration of glucose or total sugars originally contained in CWDHPs (prior to pretreatment).
^a Means within columns with unlike letters differ (P < 0.05).
NS not significant; † P < 0.1; * P < 0.05; ** P < 0.01, *** P < 0.001.

ISSN: 2731-3654