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Table 3 Enzymatic and alkali-soluble (AS) glucose and total sugars released from cell wall dehydrogenation polymers (CWDHPs)

From: Epigallocatechin gallate incorporation into lignin enhances the alkaline delignification and enzymatic saccharification of cell walls

CWDHP Alkaline pretreatment (°C) Enzymatic (%)1 Enzymatic (%)2 Enzymatic + AS (%)2
   Glucose Total sugars Glucose Total sugars Glucose Total sugars
Control None 37.9d 26.4d 37.9c 26.4bc 37.9e 26.4d
  70 60.7c 45.9c 46.1b 34.1ab 70.2d 59.9c
  100 66.6bc 52.3bc 44.0bc 32.2ab 77.9c 70.6b
  130 73.0b 54.9bc 40.3bc 28.3abc 85.1b 76.8b
EGCG None 42.5d 30.2d 42.5bc 30.2abc 42.5e 30.2d
  70 72.9b 56.1b 55.6a 36.5a 79.3bc 71.6b
  100 86.3a 67.3a 44.9bc 27.4bc 92.9a 86.7a
  130 92.4a 76.2a 38.0c 22.1c 96.9a 93.1a
Analysis of variance       
CWDHP *** * NS NS *** *
Pretreatment *** ** * NS *** ***
CWDHP X Pretreatment Interaction * * * *** *
  1. The CWDHPs were formed with monolignols (control) or with monolignols plus epigallocatechin gallate (EGCG) and pretreated with 0.075 M sodium hydroxide (15% w/w loading) at various temperatures. Data are averaged over enzymatic hydrolyses conducted for 6, 12, and 24 h.
  2. 1 Based on the concentration of glucose or total sugars in CWDHPs or their alkaline insoluble residues.
  3. 2 Based on the concentration of glucose or total sugars originally contained in CWDHPs (prior to pretreatment).
  4. a Means within columns with unlike letters differ (P < 0.05).
  5. NS not significant; † P < 0.1; * P < 0.05; ** P < 0.01, *** P < 0.001.